遗传学报
遺傳學報
유전학보
ACTA GENETICA SINICA
2000年
12期
1084-1093
,共10页
赵佐成%沈国坤%周明德%罗定泽%侯鑫%李发良%曹吉祥%CAO Ji-Xiang
趙佐成%瀋國坤%週明德%囉定澤%侯鑫%李髮良%曹吉祥%CAO Ji-Xiang
조좌성%침국곤%주명덕%라정택%후흠%리발량%조길상%CAO Ji-Xiang
苦荞麦%等位酶%遗传资源%遗传多样性
苦蕎麥%等位酶%遺傳資源%遺傳多樣性
고교맥%등위매%유전자원%유전다양성
Fagopyrum tataricum (L.) Gaertn%allozyme%genetic resource%genetic diversity
用等位酶电泳技术测定了四川省凉山彝族自治州北部越西和甘洛2县的苦荞麦[Fagopyrum tataricum (L.) Gaertn.]17个栽培居群的遗传多样性和分化,结合农业生物学性状进行了分析。对7个酶系统的15个位点的检测表明,苦荞麦居群内的遗传多样性水平高于该州南部的苦荞麦和其他地区甜荞麦,每个位点的等位基因平均数为1.9,多态位点比率为52.1%,平均实际杂合度和预期杂合度分别为0.190和0.262, FST值为0.199,居群间存在着较明显的遗传分化,接近野生植物的平均水平。对苦荞麦种质资源保护提出了以下策略:“有翅荞”2个居群的多态位点比率P为60.0%,它们的实际杂合度均高于预期杂合度,分别为Ho=0.260和Ho=0.301,是遗传多样性最丰富的居群,应重点保护。此外,遗传多样性较高的2个“小米荞”居群和遗传位置独特的“秋苦荞”、“额曲苦荞”和“革洛苦荞”的3个居群也应加强保护。
用等位酶電泳技術測定瞭四川省涼山彝族自治州北部越西和甘洛2縣的苦蕎麥[Fagopyrum tataricum (L.) Gaertn.]17箇栽培居群的遺傳多樣性和分化,結閤農業生物學性狀進行瞭分析。對7箇酶繫統的15箇位點的檢測錶明,苦蕎麥居群內的遺傳多樣性水平高于該州南部的苦蕎麥和其他地區甜蕎麥,每箇位點的等位基因平均數為1.9,多態位點比率為52.1%,平均實際雜閤度和預期雜閤度分彆為0.190和0.262, FST值為0.199,居群間存在著較明顯的遺傳分化,接近野生植物的平均水平。對苦蕎麥種質資源保護提齣瞭以下策略:“有翅蕎”2箇居群的多態位點比率P為60.0%,它們的實際雜閤度均高于預期雜閤度,分彆為Ho=0.260和Ho=0.301,是遺傳多樣性最豐富的居群,應重點保護。此外,遺傳多樣性較高的2箇“小米蕎”居群和遺傳位置獨特的“鞦苦蕎”、“額麯苦蕎”和“革洛苦蕎”的3箇居群也應加彊保護。
용등위매전영기술측정료사천성량산이족자치주북부월서화감락2현적고교맥[Fagopyrum tataricum (L.) Gaertn.]17개재배거군적유전다양성화분화,결합농업생물학성상진행료분석。대7개매계통적15개위점적검측표명,고교맥거군내적유전다양성수평고우해주남부적고교맥화기타지구첨교맥,매개위점적등위기인평균수위1.9,다태위점비솔위52.1%,평균실제잡합도화예기잡합도분별위0.190화0.262, FST치위0.199,거군간존재착교명현적유전분화,접근야생식물적평균수평。대고교맥충질자원보호제출료이하책략:“유시교”2개거군적다태위점비솔P위60.0%,타문적실제잡합도균고우예기잡합도,분별위Ho=0.260화Ho=0.301,시유전다양성최봉부적거군,응중점보호。차외,유전다양성교고적2개“소미교”거군화유전위치독특적“추고교”、“액곡고교”화“혁락고교”적3개거군야응가강보호。
In the present paper is stated the accomplishment of the studies of 17 populations of cultivated Fagopyrum tataricum (L.) Gaertn. from the counties of Yuexiand Ganluo in North Liangshan Autonomous Prefecture of Yi Nationality, Sichuan Province, China by means of strarch gel electrophoresis and cluster analysis of agrobiology features. Seven enzymes, and 15 loci were assessed. The result shows that the genetic diversity of F. tataricum within population is higher than in the South Prefecture and in species F. esculentum from other areas. The A, P, Ho and He are 1.9, 52.1%, 0.190 and 0.262 respectively. The FST is 0.199 which is close to the average level of wild plants. A conservation strategy of the genetic resource of F. tataricum is suggested. Two populations of local variety “Youchiqiao” have the richest genetic diversity, P=60.0%, Ho=0.260 and 0.301 and they should be protected particularly. Two populations of local variety“Xiaomiqiao” with higher genetic diversity,as well as three populations of local varieties of “Qiukuqiao”, “Equkuqiao” and “Geluokuqiao” with special genetic position should be protected too.