CAJ | 학술논문

根据敌百虫与苏云金杆菌(Bt)混用具有显著增效作用的结果，分别用Bt 240 mg*L－1，敌百虫90 mg*L－1和两者的混剂(23 mg*L －1敌百虫加57 mg*L－1 Bt或90 mg*L－1敌百虫加225 mg* L－1 Bt)处理棉铃虫5龄幼虫，测定其对活体棉铃虫幼虫乙酰胆碱酯酶的影响。结果表明：若以每个头部计算，Bt单用并不影响乙酰胆碱酯酶，但与敌百虫混用时，能增加敌百虫对乙酰胆碱酯酶的抑制作用。此外，Bt处理显著降低了幼虫头内蛋白质含量，处理后1，2 ，3和4 d，蛋白质含量分别是对照的85%，79%，73%和72%。基于以上结果，讨论了两者混用增效的途径。
근거활백충여소운금간균(Bt)혼용구유현저증효작용적결과，분별용Bt 240 mg*L－1，활백충90 mg*L－1화량자적혼제(23 mg*L －1활백충가57 mg*L－1 Bt혹90 mg*L－1활백충가225 mg* L－1 Bt)처리면령충5령유충，측정기대활체면령충유충을선담감지매적영향。결과표명：약이매개두부계산，Bt단용병불영향을선담감지매，단여활백충혼용시，능증가활백충대을선담감지매적억제작용。차외，Bt처리현저강저료유충두내단백질함량，처리후1，2 ，3화4 d，단백질함량분별시대조적85%，79%，73%화72%。기우이상결과，토론료량자혼용증효적도경。
Based on the synergism of the mixture of Bt and tri chlorphon,experiment was carried out to declare if AChE was involved in the syne rgistic mechanism.The fifth instar larvae of cotton bollworm were treated with 9 0 mg*L－1 trichlorphon,240 mg*L－1 Bt and mixtures contained 23 mg *L－1 trichlorphon and 57 mg*L－1 Bt or 90 mg*L－1 trichlorphon and 225 mg*L－1 Bt (Treatment 1,2,3 and 4).The activity of acetylcholinesterase(AChE)was analyzed in viro.It was found that Bt exerted little effect to the activity of AChE when used alone but it enhanced the inhibition of trichlorphon to AChE when used in mixture.Furthermore,protein content in larva′s head decreased dramatically after treated with Bt.When tested in 1,2,3 or 4 days,there was only 85%,79%,73% or 72% of that in CK,respective ly.The means of joint effect of Bt and trichlorphon on AChE were finally discussed.