CAJ | 학술논문

研究了胸腺嘧啶核苷(TdR)双阻断法同步化Vero细胞的最适条件，包括TdR处理浓度、TdR处理时间和两次阻断间的恢复生长时间. 结果表明，在指数生长前期的细胞，用2 mmol/L的TdR阻断细胞生长11 h，恢复生长14 h，再用2 mmol/L的TdR阻断11 h，所得Vero细胞的同步化程度最高. 用该方法分别处理在方瓶中贴壁生长的Vero细胞和旋转培养瓶中用Cytodex-3培养的Vero细胞，其同步化指数分别为65.0%和60.0%.
연구료흉선밀정핵감(TdR)쌍조단법동보화Vero세포적최괄조건，포괄TdR처리농도、TdR처리시간화량차조단간적회복생장시간. 결과표명，재지수생장전기적세포，용2 mmol/L적TdR조단세포생장11 h，회복생장14 h，재용2 mmol/L적TdR조단11 h，소득Vero세포적동보화정도최고. 용해방법분별처리재방병중첩벽생장적Vero세포화선전배양병중용Cytodex-3배양적Vero세포，기동보화지수분별위65.0%화60.0%.
The optimum conditions of synchronous growth of Vero cells with double thymidine (TdR) block were investigated, including the concentration of TdR, treatment time and recovery time. The proper operation procedure is as follows: exponentially growing Vero cells were arrested with 2 mmol/L TdR for 11 h, cultured in normal medium for 14 h and arrested with TdR for another 11 h. In this way, the Synchronous Index (SI) reached 65.0% for the Vero cells culture in bottles and 60.0% for Vero cells growing on Cytodex-3 microcarriers in spinner flasks.