CAJ | 학술논문

以甘蓝型油菜显性核不育杂合两型系的不育株沪油15A为母本,与恢复系HF03杂交,F_1群体全部表现为可育.在该杂交组合的6个F_3群体中有5个群体发生育性分离.其中区号为04-4307的F_3群体发生育性和花瓣色泽的分离,表现为70株鲜黄花可育、22株鲜黄花不育、30株橙黄花可育、9株橙黄花不育.根据油菜显性核不育和橙黄花的遗传规律,04-4307出现3∶1育性分离,说明该群体由基因型为MsMsRfrf的单株自交获得.从04-4307群体中选橙黄花可育株自交,并与橙黄花不育株作兄妹交,后代全部表现为橙黄花(y1y1y2y2).其中区号为05-4356的兄妹交组合出现1∶1育性分离;其父本的可育株自交出现3∶1育性分离.由此推断,05-4356的兄妹交组合就是显性核不育橙黄花纯合两型系,其不育株基因型为MsMsrfrfy1y1y2y2,可育株基因型为MsMsRfrfy1y1y2y2.
이감람형유채현성핵불육잡합량형계적불육주호유15A위모본,여회복계HF03잡교,F_1군체전부표현위가육.재해잡교조합적6개F_3군체중유5개군체발생육성분리.기중구호위04-4307적F_3군체발생육성화화판색택적분리,표현위70주선황화가육、22주선황화불육、30주등황화가육、9주등황화불육.근거유채현성핵불육화등황화적유전규률,04-4307출현3∶1육성분리,설명해군체유기인형위MsMsRfrf적단주자교획득.종04-4307군체중선등황화가육주자교,병여등황화불육주작형매교,후대전부표현위등황화(y1y1y2y2).기중구호위05-4356적형매교조합출현1∶1육성분리;기부본적가육주자교출현3∶1육성분리.유차추단,05-4356적형매교조합취시현성핵불육등황화순합량형계,기불육주기인형위MsMsrfrfy1y1y2y2,가육주기인형위MsMsRfrfy1y1y2y2.
A cross between heterozygous two-type line Huyou15A of dominant genic male sterility and restorer line HF03 led to all fertile F_1 plants. Five among 6 F_3 generation lines showed fertile segregation.A registered 04-4307 group showed a fertility segregation ratio at 3∶1,including 70 fertile with yellow flowers,30 fertile with orange flowers,22 sterile with yellow flowers and 9 sterile with orange flowers.The genotype of its F_2 plant should be MsMsRfrf. The fertile plants with orange flowers were self-pollinated and crossed with sister sterile plants with orange flowers. Both led to all orange flower offspring, while the sib-mating generation 05-4356 showed a fertility segregation ratio at 1∶1, and the self generation of corresponding fertile plant segregated at 3∶1.These results indicated the 05-4356 group to be a homozygous two-type line with orange flower developed by crossing sterile genotype MsMsrfrfy1y1y2y2 and fertile genotype MsMsRfrfy1y1y2y2.