CAJ | 학술논문

以乙醇为浸提溶剂,超声波辅助提取不同品种大豆中的木脂素,并用紫外-可见分光光度法对其含量进行分析,同时对浸提溶剂、料液比、浸提时间及次数等因素进行比较.结果表明:以乙醇为浸提溶剂,料液比在1/8～1/12范围,浸提时间20 min,浸提2次效果比较理想;标准对照品在0～0.18 mg · mL~(-1)浓度范围内吸光度与其总木脂素含量呈良好的线性关系,回归方程A=6.081c+0.0445,相关系数r=0.9998,总木脂素检出限8.3×10~(-5) mg · mL~(-1),平均回收率为94.4%,RSD为1.2%.该方法简便、实用、可靠,可对大豆木脂素的深入研究提供参考.
이을순위침제용제,초성파보조제취불동품충대두중적목지소,병용자외-가견분광광도법대기함량진행분석,동시대침제용제、료액비、침제시간급차수등인소진행비교.결과표명:이을순위침제용제,료액비재1/8～1/12범위,침제시간20 min,침제2차효과비교이상;표준대조품재0～0.18 mg · mL~(-1)농도범위내흡광도여기총목지소함량정량호적선성관계,회귀방정A=6.081c+0.0445,상관계수r=0.9998,총목지소검출한8.3×10~(-5) mg · mL~(-1),평균회수솔위94.4%,RSD위1.2%.해방법간편、실용、가고,가대대두목지소적심입연구제공삼고.
Total lignans in several soybean samples were detected by UV-Vis with the ultrasonic-assisted extraction in this paper.The result showed that by twice extraction it can get ideal effect with ethanol as extracting reagent in the material/reagent ratio of 1/8～1/12.The relationship between standard substance concentration within 0～0 18 mg · mL~(-1) and absorbance remained better linear.The regression equation was A=6.081c+0.0445,with the correlation coefficient r=0 9998,the detection limit was 8.3×10~(-5) mg · mL~(-1)with the average recovery of 94.4% and the RSD of 1.2%.Results suggest the method is convenient and practical to detect lignans from soybean.