CAJ | 학술논문

Harpins是由革兰氏阴性植物病原细菌产生的一类蛋白激发子,对蛋白酶敏感,在非寄主植物叶片上可以诱导hypersensitive response (HR)或hypersensitive cell death (HCD).HrpN_Ea是Harpins蛋白的一种,是从梨火疫病菌(Erwinia amylovora)中分离得到的.大量试验证明,HrpN_Ea蛋白在引起植物过敏反应的同时,能诱导植物获得性系统抗性及刺激植物生长发育.从分子生物学角度,利用国际互联网上的相关生物信息学分析软件NPSA、Swiss-Model、SAPS、InterPro Scan等对HrpN_Ea蛋白进行了结构和功能的预测分析.HrpN_Ea蛋白由403个氨基酸组成,有较多的β折叠和无规卷曲结构;HrpN_Ea蛋白无N端信号肽,不形成跨膜结构,定位于细胞质中.
Harpins시유혁란씨음성식물병원세균산생적일류단백격발자,대단백매민감,재비기주식물협편상가이유도hypersensitive response (HR)혹hypersensitive cell death (HCD).HrpN_Ea시Harpins단백적일충,시종리화역병균(Erwinia amylovora)중분리득도적.대량시험증명,HrpN_Ea단백재인기식물과민반응적동시,능유도식물획득성계통항성급자격식물생장발육.종분자생물학각도,이용국제호련망상적상관생물신식학분석연건NPSA、Swiss-Model、SAPS、InterPro Scan등대HrpN_Ea단백진행료결구화공능적예측분석.HrpN_Ea단백유403개안기산조성,유교다적β절첩화무규권곡결구;HrpN_Ea단백무N단신호태,불형성과막결구,정위우세포질중.
Harpins produced by gram-negative plant pathogenic bacteria,a class of elicitor proteins,can induce hypersensitive response (HR) or hypersensitive cell death (HCD) in non-host plant leaves.HrpN_Ea is one kind of Harpins.It is separated from Erwinia amylovora.A large number of experiments proved that HrpN_Ea causes hypersensitive reactions in plants,also can induce acquired systemic resistance of plants and stimulate plant growth and development at the same time.The study analyzed protein structure and function of HrpN_Ea from the perspective of molecular biology by using bioinformatics software such as NPSA,Swiss-Model,SAPS,InterPro Scan and so on.HrpN_Ea is composed of 403 amino acids,and it has more β-sheet and random coil structure; HrpN_Ea protein without N-signal peptide,does not form a transmembrane structure,located in the cytoplasm.