CAJ | 학술논문

旨在建立有效扩增16S rRNA基因序列的放线菌DNA快速提取的方法.采用Chelex-100法提取放线菌DNA,使用PCR扩增16S rRNA基因序列评价提取核酸的质量.结果显示,Chelex-100法能够在10 min之内从放线菌中快速提取DNA,所提取的DNA可以直接用于PCR扩增反应,PCR扩增产物电泳条带清晰,符合理论预期结果.因此,Chelex-100法提取放线菌DNA可以作为16S rRNA基因序列PCR扩增的模板,该方法具有经济、简便、快速的特点,适合于放线菌菌株大规模地筛选和分类鉴定.
지재건립유효확증16S rRNA기인서렬적방선균DNA쾌속제취적방법.채용Chelex-100법제취방선균DNA,사용PCR확증16S rRNA기인서렬평개제취핵산적질량.결과현시,Chelex-100법능구재10 min지내종방선균중쾌속제취DNA,소제취적DNA가이직접용우PCR확증반응,PCR확증산물전영조대청석,부합이론예기결과.인차,Chelex-100법제취방선균DNA가이작위16S rRNA기인서렬PCR확증적모판,해방법구유경제、간편、쾌속적특점,괄합우방선균균주대규모지사선화분류감정.
To establish a rapid method for extracting DNA from actinomycetes,DNA was extracted from actinomycetes by Chelex100.The quality of the extraction by 16S rRNA gene was evaluated with PCR technique.The PCR product of 16S rRNA gene was used as a mark fragment.Results showed the Chlex-100 can quickly extract the DNA from actinomycetes about in 10 min.The DNA can be directly applied to the following step-PCR amplification as DNA template.Electrophoresis results of the PCR products was clear.Therefore,Chelex-100 extraction method could be an efficient and reliable method for PCR detection and used in actinomycetes screening and identification rapidly.