CAJ | 학술논문

目的制备水溶性CdTe量子点-广谱抗细胞角蛋白单克隆抗体(QDs-MAb)荧光探针,并对其特异免疫性识别能力和荧光稳定性进行检测.方法在EDC偶联剂的作用下,将水溶性CdTe量子点与广谱细胞角蛋白单克隆抗体(Pan CK)进行了连接;对肝癌细胞HepG2采用免疫细胞化学法和QDs-MAb单抗荧光探针直接免疫荧光标记法观察比较Pan CK蛋白在细胞内的分布;将量子点与传统的荧光染料FITC的荧光强度和荧光稳定性进行了比较.结果 QDs-MAb单抗荧光探针对HepG2细胞内的Pan CK分子具有特异性的识别能力;与FITC相比,QDs-MAb荧光探针荧光度更强,光化学稳定性更好,激发光连续照射30 min及室温放置3 d后均未见明显淬灭.结论本实验成功制备了QDs-MAb荧光探针,为半导体量子点用于上皮来源的肿瘤细胞内Pan CK分子的相关检测提供了科学依据.
목적 제비수용성CdTe양자점-엄보항세포각단백단극륭항체(QDs-MAb)형광탐침,병대기특이면역성식별능력화형광은정성진행검측.방법 재EDC우련제적작용하,장수용성CdTe양자점여엄보세포각단백단극륭항체(Pan CK)진행료련접;대간암세포HepG2채용면역세포화학법화QDs-MAb단항형광탐침직접면역형광표기법관찰비교Pan CK단백재세포내적분포;장양자점여전통적형광염료FITC적형광강도화형광은정성진행료비교.결과 QDs-MAb단항형광탐침대HepG2세포내적Pan CK분자구유특이성적식별능력;여FITC상비,QDs-MAb형광탐침형광도경강,광화학은정성경호,격발광련속조사30 min급실온방치3 d후균미견명현쉬멸.결론 본실험성공제비료QDs-MAb형광탐침,위반도체양자점용우상피래원적종류세포내Pan CK분자적상관검측제공료과학의거.
Objective To prepare semiconductor quantum dots(QDs)-Pan CK monoclonal antibody fluorescent probes and then to detect the ability to specific recognition of Pan CK in HepG2 cells. Methods QDs-Pan CK fluorescent probes were prepared by linking water soluble CdTe and Pan CK monoclonal antibody through EDC coupling reagents and then the probes were purified. The expression of Pan CK protein in HepG2 cells was observed by immunocytochemistry and direct immunofluorescence labeling by QDs-Pan CK, and was compared with FITC indirect immunofluorescence labeling. Results Water soluble CdTe and Pan CK monoclonal antibody formed stable fluorescent probes through covalent bond and the probe could recognize specifically Pan CK protein in HepG2 cells and were superior to traditional organic fluorescence dyes in sensitivity and photostability.The signals of QDs showed no obvious change during the continuous illumination by excition light for 30min and after 3 d preserved in room temperature. Conclusions QDs-Pan CK fluorescent probes are prepared successfully which provide new methods for diagnosis and prognosis for epithelial-derived tumor.