海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2010年
8期
252-254
,共3页
环抱菌素A%发酵%优化%均匀设计
環抱菌素A%髮酵%優化%均勻設計
배포균소A%발효%우화%균균설계
利用"单因素多浓度"和"均匀设计法"对环抱菌素生产菌株茄病镰刀菌的发酵条件和发酵培养基组分进行优化,并在5000L发酵罐上对菌株的发酵动力学规律作了初步的探索.实验结果表明,5.5%玉米粉,0.5%葡萄糖,0.5%干酪素,0.05%KCI,0.05%MgSO4,0.015%KH2PO4和0.3%CaCO3为最佳摇瓶发酵培养基组成;5000L发酵罐的发酵动力学规律表明,菌体浓度发酵培养至80h左右达到最大,产素水平在116h左右达到最大.
利用"單因素多濃度"和"均勻設計法"對環抱菌素生產菌株茄病鐮刀菌的髮酵條件和髮酵培養基組分進行優化,併在5000L髮酵罐上對菌株的髮酵動力學規律作瞭初步的探索.實驗結果錶明,5.5%玉米粉,0.5%葡萄糖,0.5%榦酪素,0.05%KCI,0.05%MgSO4,0.015%KH2PO4和0.3%CaCO3為最佳搖瓶髮酵培養基組成;5000L髮酵罐的髮酵動力學規律錶明,菌體濃度髮酵培養至80h左右達到最大,產素水平在116h左右達到最大.
이용"단인소다농도"화"균균설계법"대배포균소생산균주가병렴도균적발효조건화발효배양기조분진행우화,병재5000L발효관상대균주적발효동역학규률작료초보적탐색.실험결과표명,5.5%옥미분,0.5%포도당,0.5%간락소,0.05%KCI,0.05%MgSO4,0.015%KH2PO4화0.3%CaCO3위최가요병발효배양기조성;5000L발효관적발효동역학규률표명,균체농도발효배양지80h좌우체도최대,산소수평재116h좌우체도최대.
