CAJ | 학술논문

近年来,随着微操作研究的不断发展和深入,批量目标微操作成为当前的研究热点之一.由于显微镜视野范围狭小,微操作过程中需要不断进行物镜转换和调焦工作.本文研制的全自动微操作平台将调焦和物镜转换改造为电动方式,实现了自动调焦和物镜自动转换功能.针对细胞图像的特点,本文提出了基于扫描线的归一化方差聚焦测度方法,并在此基础上,设计了相应的聚焦平面搜索策略.针对显微镜物镜转化器的特点,本文设计了自动物镜转换结构和控制方法,实现不同放大倍数物镜的转换.最后本文在细胞图像处理的实验中,构建了具有分层递阶结构的全局视野,验证了自动调焦和物镜自动转换的准确控制,为批量目标操作的自动化奠定了基础.
근년래,수착미조작연구적불단발전화심입,비량목표미조작성위당전적연구열점지일.유우현미경시야범위협소,미조작과정중수요불단진행물경전환화조초공작.본문연제적전자동미조작평태장조초화물경전환개조위전동방식,실현료자동조초화물경자동전환공능.침대세포도상적특점,본문제출료기우소묘선적귀일화방차취초측도방법,병재차기출상,설계료상응적취초평면수색책략.침대현미경물경전화기적특점,본문설계료자동물경전환결구화공제방법,실현불동방대배수물경적전환.최후본문재세포도상처리적실험중,구건료구유분층체계결구적전국시야,험증료자동조초화물경자동전환적준학공제,위비량목표조작적자동화전정료기출.
With the development of micro-manipulation techniques, batch-targets-oriented operation has become the focus in the research. During batch-targets-oriented micro-manipulation, the objective lens and focused position need to be adjusted from time to time because the view field of microscope is very limited. This paper introduced an automated micro-manipulation platform with auto-focusing and auto-switching of the objective lens to realize batch-targets-oriented manipulation. An improved normalized variance algorithm based on scanning lines was presented to save time for online calculation. A searching strategy based on the algorithm was designed to realize the auto-focusing under any condition. A set of special mechanism of auto-switching of the objective lens was introduced with the control method. Finally, in the experiment of cell image processing, the global field of view (FOV) of the hierarchical structure was constructed, which is the foundation of batch-targets-oriented micro-manipulation. The test result verifies the validity and correctness of the improvement in the microscope's visual system.