CAJ | 학술논문

根据经典微生物划线纯培养方法从湛江一个盐田泥样中分离获得1个嗜盐碱古菌菌株SCSIO CD13T,通过形态、细胞化学分类及分子发育等对其进行系统分类鉴定研究.经鉴定该菌细胞球形,好氧,革兰染色不定,在最适pH 8.5和37℃培养条件下,能够耐受2.5～5.5 mol/L盐浓度生长.16S rRNA基因序列比对分析显示,该菌株与嗜盐碱古菌Natronococcus属成员N.jeotgali B1T(99.2％),N.occultus NCMB 2192T (96.8％)和N.amylolyticus Ah-36T(95.8％)最相近,且系统发育树和细胞化学特征与Natronococcus属相一致.然而,系统发育树上显示该菌株与最相似菌种形成单独分支,且生理学特性显示出与最相似的3个物种具有明显的差异,包括盐浓度、pH耐受,镁离子需求,H2S产生,淀粉、明胶水解,抗生素敏感性等.另外,杂交结果显示,菌株SCSIO CD13T与最相似菌种Natronococcus jeotgali B1T的杂交值为23％；综合多相分类结果,菌株SCSIO CD13T应为Natronococcus属的一个新成员,将其命名为湛江嗜盐碱球菌新种(Natronococcus zhanjiangensis),典型菌株SCSIO CD13T.
근거경전미생물화선순배양방법종담강일개염전니양중분리획득1개기염감고균균주SCSIO CD13T,통과형태、세포화학분류급분자발육등대기진행계통분류감정연구.경감정해균세포구형,호양,혁란염색불정,재최괄pH 8.5화37℃배양조건하,능구내수2.5～5.5 mol/L염농도생장.16S rRNA기인서렬비대분석현시,해균주여기염감고균Natronococcus속성원N.jeotgali B1T(99.2％),N.occultus NCMB 2192T (96.8％)화N.amylolyticus Ah-36T(95.8％)최상근,차계통발육수화세포화학특정여Natronococcus속상일치.연이,계통발육수상현시해균주여최상사균충형성단독분지,차생이학특성현시출여최상사적3개물충구유명현적차이,포괄염농도、pH내수,미리자수구,H2S산생,정분、명효수해,항생소민감성등.령외,잡교결과현시,균주SCSIO CD13T여최상사균충Natronococcus jeotgali B1T적잡교치위23％；종합다상분류결과,균주SCSIO CD13T응위Natronococcus속적일개신성원,장기명명위담강기염감구균신충(Natronococcus zhanjiangensis),전형균주SCSIO CD13T.
A novel halophilic archaeon,strain CD13T,was isolated from saltern sediment of Zhan Jiang,Guangdong Province,China,by using classical spread plate method.And it was characterized on the basis of phenotypic,chemotaxonomic and phylogenetic features.Results showed that the cells of strain CD13T,were aerobic,gram-variable and cocci.It could grow at 2.5 ～ 5.5 mol/L NaC1 with the optima of pH 8.5 and 37 C.Magnesium was not required for growth.Partial 16S rRNA gene sequence analysis showed that the isolate was closest to Natronococcus jeotgali B1T (99.2％,sequence similarity),Natronococcus occultus NCMB 2192T(96.8％)and Natronococcus amylolyticus Ah36T(95.8％).The constructed phylogenetic tree based on 16S rRNA gene sequences showed that t strain CD13T formed a distinct cluster with N.jeotgali B1 T,N.occultus NCMB 2192T and N.amylolyticus Ah-36T.In addition,chemotaxonomic properties including polar lipids and menaquinones componentes are consistent with those of the genus Natronococcus.However,strain SCSIO CD13T could be differentiated from its closest phylogenetic neighbours by physiological and chemical characteristics.Furthermore,DNA-DNA hybridization level between strain CD13T and its closest phylogenetic neighbor Ncc jeotgali B1T was 23％,which was far below the 70％ cut-off point.Based on the polyphasic taxonomic data,strain SCSIO CD13T represents a hitherto unknown species of the genus Natronococcus,for which the name Natronococcus zhanjiangensis sp.nov,was proposed.The type strain is SCSIO CD13T.