CAJ | 학술논문

目的构建人生长激素的真核细胞表达载体,测定并分析目的基因序列.方法用PCR方法扩增出人生长激素基因,连接至T克隆载体,然后分别亚克隆至真核细胞表达载体pCDNA3.1、pCEP4中.使用DNA ssist软件分析序列.结果所构建真核表达载体的酶切鉴定、PCR鉴定、测序鉴定均正确.结论成功构建出多个人生长激素基因真核细胞表达载体,验证了基因序列的正确性,为下一步基因治疗研究打下基础.
목적 구건인생장격소적진핵세포표체재체,측정병분석목적 기인서렬.방법 용PCR방법확증출인생장격소기인,련접지T극륭재체,연후분별아극륭지진핵세포표체재체pCDNA3.1、pCEP4중.사용DNA ssist연건분석서렬.결과 소구건진핵표체재체적매절감정、PCR감정、측서감정균정학.결론 성공구건출다개인생장격소기인진핵세포표체재체,험증료기인서렬적정학성,위하일보기인치료연구타하기출.
Obiective To construct eukaratic expression vector for human growth hormone gene (hGH) and analyze the sequence for further research work. Methods hGH gene was obtained from pNMG3 with PCR, and ligated into pMD-18-T clone vector, then subcloned into another two expression vector pCD-NA3. 1 and pCEP4 selectively. The sequence was analyzed with software and compared with the open data in Genebank. Results The constructed vectors were identified with enzyme cutting, PCR, and finally with gene sequencing. The hGH gene of pCDNA3. 1-hGH and pCEP4-hGH was the same as pNMG3. However, it was different from the open data of hGH gene in Genebank, and the difference was introns of hGH gene. Conclusion hGH gene in the potent vector - pNMG3 was subcloned into the other two more powerful eukaryotic expression vectors successfully. It is essential for the later gene therapy work to select out an ideal vector for hGH gene.