中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
2期
153-154
,共2页
刘爱杰%杨小霖%张文胜%马汉祥%杨宗斌%饶艳%杨经文%罗南富%李杰%刘进
劉愛傑%楊小霖%張文勝%馬漢祥%楊宗斌%饒豔%楊經文%囉南富%李傑%劉進
류애걸%양소림%장문성%마한상%양종빈%요염%양경문%라남부%리걸%류진
异氟醚%急性毒性试验%剂量效应关系,药物
異氟醚%急性毒性試驗%劑量效應關繫,藥物
이불미%급성독성시험%제량효응관계,약물
Isoflurane%Acute toxicity tests%Dose-response relationship,drug
目的 研究大鼠尾静脉注射乳化异氟烷的急性毒性并确定其半数致死剂量(LD_(50)).方法 清洁级SD大鼠60只,按雌雄分层随机分为6组,溶媒对照组(C组)和不同剂量乳化异氟烷组(I_(1～5)组),每组10只,雌雄各半.I_(1～5)组分别单次尾静脉注射乳化异氟烷225.6、192.0、163.2、139.2、118.8 mg/ks,以心电图成一直线判断死亡,采用Bliss法计算乳化异氟烷的LD_(50)及其95%可信区间.C组单次尾静脉注射等容积30%脂肪乳.注射乳化异氟烷后观察2周.结果 大鼠单次尾静脉注射乳化异氟烷后出现翻正反射消失、呼吸抑制、心律失常或死亡;乳化异氟烷的LD_(50)为170.76 mg/kg,95%可信区间为153.00～190.56 mg/kg.结论 大鼠单次尾静脉注射乳化异氟烷的LD_(50)为170.76 mg/kg,急性毒性主要表现为呼吸循环抑制.
目的 研究大鼠尾靜脈註射乳化異氟烷的急性毒性併確定其半數緻死劑量(LD_(50)).方法 清潔級SD大鼠60隻,按雌雄分層隨機分為6組,溶媒對照組(C組)和不同劑量乳化異氟烷組(I_(1～5)組),每組10隻,雌雄各半.I_(1～5)組分彆單次尾靜脈註射乳化異氟烷225.6、192.0、163.2、139.2、118.8 mg/ks,以心電圖成一直線判斷死亡,採用Bliss法計算乳化異氟烷的LD_(50)及其95%可信區間.C組單次尾靜脈註射等容積30%脂肪乳.註射乳化異氟烷後觀察2週.結果 大鼠單次尾靜脈註射乳化異氟烷後齣現翻正反射消失、呼吸抑製、心律失常或死亡;乳化異氟烷的LD_(50)為170.76 mg/kg,95%可信區間為153.00～190.56 mg/kg.結論 大鼠單次尾靜脈註射乳化異氟烷的LD_(50)為170.76 mg/kg,急性毒性主要錶現為呼吸循環抑製.
목적 연구대서미정맥주사유화이불완적급성독성병학정기반수치사제량(LD_(50)).방법 청길급SD대서60지,안자웅분층수궤분위6조,용매대조조(C조)화불동제량유화이불완조(I_(1～5)조),매조10지,자웅각반.I_(1～5)조분별단차미정맥주사유화이불완225.6、192.0、163.2、139.2、118.8 mg/ks,이심전도성일직선판단사망,채용Bliss법계산유화이불완적LD_(50)급기95%가신구간.C조단차미정맥주사등용적30%지방유.주사유화이불완후관찰2주.결과 대서단차미정맥주사유화이불완후출현번정반사소실、호흡억제、심률실상혹사망;유화이불완적LD_(50)위170.76 mg/kg,95%가신구간위153.00～190.56 mg/kg.결론 대서단차미정맥주사유화이불완적LD_(50)위170.76 mg/kg,급성독성주요표현위호흡순배억제.
Objective To investigate the acute toxicity of intravenous isoflurane and determine the median lethal dose(LD_(50))in rats.Methods Sixty SD rats were randomly divided into control group(n = 10)and different doses of isoflurane group(I_(1-5) group,n = 10,5 male,5 female).The I_(1-5) group received a bolus of intravenous isoflurane 225.6,192.0,163.2,139.2 and 118.8 mg/kg injected via tail vein over 10 seconds respectively and LD_(50) was calculated by Bliss.In control group 30% lipid emulsion was administered instead of isoflurane.The survived rats were observed for two weeks.Results The animals exhibited loss of righting reflex,cardio-respiratory depression or death.The LD_(50) of intravenous isoflurane was 170.76 mg/kg and 95% confidence interval was 153.00-190.56 mg/kg.Conclusion The LD_(50) of intravenous isoflurane was 170.76 mg/kg,and the acute toxicity of intravenous isoflurane is cardio-respiratory depression.
