CAJ | 학술논문

目的分析年轻妇女宫颈癌人类白细胞抗原(HLA)-DRB1与-DQBI等位基因的多态性分布及与人乳头瘤病毒(HPV)16亚型感染的关系.方法选择温州医学院附属第一医院妇产科2005年1月至2009年8月诊治的166例官颈鳞状上皮癌患者,分为低龄组(年龄≤35岁,59例),高龄组(107例).以同期因子宫肌瘤收治患者共50例为正常对照组.采用聚合酶链反应检测官颈组织中HPV16感染情况,同时采用多聚酶链反应-序列特异性引物方法检测患者HLA-DRB1与-DRQ1等位基因,计算等位基因频率.结果 (1)HLA-DQB1*0301亚型基因频率在HPV16阳性的低龄宫颈癌患者中为29.6%,高龄组为12.9%,差异有统计学意义(P<0.05).HLA-DRB1*04、-DRB1*09亚型的基因频率在HPV16阴性的低龄宫颈癌患者中分别为14.1%与26.6%,高龄组为5.9%与10.5%,差异有统计学意义(P<0.05).HLA-DRB1*07亚型在HPV16阴性的低龄宫颈癌患者中未检出,而在高龄组检出14例(9.2%),差异有统计学意义(P<0.05).(2)低龄宫颈癌组HLA-DQB1*0501亚型的基因频率在HPV16阳性与阴性患者中分别为7.4%与6.3%,均明显低于高龄组的25.8%与20.4%,差异有统计学意义(P<0.05).结论年轻官颈癌患者某些HLA-DRB1、-DQB1等位基因亚型分布不同于年长者,并且与其HPV16感染状态存在一定关系.
목적 분석년경부녀궁경암인류백세포항원(HLA)-DRB1여-DQBI등위기인적다태성분포급여인유두류병독(HPV)16아형감염적관계.방법 선택온주의학원부속제일의원부산과2005년1월지2009년8월진치적166례관경린상상피암환자,분위저령조(년령≤35세,59례),고령조(107례).이동기인자궁기류수치환자공50례위정상대조조.채용취합매련반응검측관경조직중HPV16감염정황,동시채용다취매련반응-서렬특이성인물방법검측환자HLA-DRB1여-DRQ1등위기인,계산등위기인빈솔.결과 (1)HLA-DQB1*0301아형기인빈솔재HPV16양성적저령궁경암환자중위29.6%,고령조위12.9%,차이유통계학의의(P<0.05).HLA-DRB1*04、-DRB1*09아형적기인빈솔재HPV16음성적저령궁경암환자중분별위14.1%여26.6%,고령조위5.9%여10.5%,차이유통계학의의(P<0.05).HLA-DRB1*07아형재HPV16음성적저령궁경암환자중미검출,이재고령조검출14례(9.2%),차이유통계학의의(P<0.05).(2)저령궁경암조HLA-DQB1*0501아형적기인빈솔재HPV16양성여음성환자중분별위7.4%여6.3%,균명현저우고령조적25.8%여20.4%,차이유통계학의의(P<0.05).결론 년경관경암환자모사HLA-DRB1、-DQB1등위기인아형분포불동우년장자,병차여기HPV16감염상태존재일정관계.
Objective To investigate the polymorphism of human leukocyte antigen (HLA)-DRB1 and-DQB1 alleles among young women with cervical squamous cell carcinoma (SCC) and elucidate its relationship with human papillomavirus (HPV) type 16 infection.Methods From January 2005 to August 2009,166 women diagnosed with cervical SCC at our hospital were enrolled.Thsse patients were divided into two groups based on age,including 59 cases in young age group ( ≤35 yrs) and 107 cases in non-young age group.In the mean time,50 cases with uterine myoma treated by hysterectomy were selected as controls.HPV 16 DNA in cervical tissues was amplified by polymerase chain reaction (PCR).HLA-DRB1 and -DQB1 typing were carried out by PCR-sequence specific primer (PCR-SSP) and the allele frequencies calculated.Results (1)The allele frequency of HLA DQB1 * 0301 at 29.6% was detected among HPV 16positive cervical SCC cases in young age group.And it was significantly higher than 12.9% of non-young age group (P<0.05 ).The allele frequencies of HLA-DRB1 * 04 and -DRB1 * 09 were significantly higher among HPV 16 negative cervical SCC cases in young age group as compared with non-young age group (14.1%,26.6% vs 5.9%,10.5%) (P<0.05).The HLA-DRB1 *07 allele was not detected among HPV 16 negative cervical SCC cases in young age group,But 14 cases (9.2%) were detected in non-young age group (P<0.05).(2)The allele frequencies of HLA-DQB1 * 0501 at 7.4% and 6.3% respectively were detected among HPV 16 positive and negative cervical SCC cases in the young age group.And they were significantly lower than 25.8% and 20.4% of non-young age group ( P<0.05 ).Conclusion The distribution patterns of HLA-DRB1 and -DQB1 alleles among young women with cervical SCC are different from those of older ones.And it has something to do with the HPV 16 infection status.