CAJ | 학술논문

目的在整体水平探讨小鼠巨细胞病毒(MCMV)感染对小鼠调节性T细胞(Treg)亚群CD4+CD25+Foxp3+T细胞分化和CD4+CD25+Foxpp3-效应性T细胞(TE)活化的影响.方法建立全身播散型MCMV感染小鼠模型,依据主要脏器内病毒滴度,确定感染后第28天为本模型急、慢性期界定点.42只小鼠分别于感染MCMV后第1、3、7、14、28、45和60天处死6只;另设42只小鼠作为对照.流式细胞术检测CD4+CD25+F0xp34+Treg和活化的CD4+CD25+Foxp3-TE在脾单个核细胞中所占比率变化.结果 MCMV感染急性期CD4+CD25+Foxp3+Treg比率持续降低,第28天左右降至最低值,与对照组比较差异有统计学意义(P<0.01);随后直线上升,第45天高于基线,第60天升至峰值,与对照组比较差异有统计学意义(P<0.05);CD4+CD25+Foxp3-TE在感染后第3～7天高于对照组(P<0.05),其后持续下降,在感染后第45天和第60天显著低于对照组水平(P<0.05).Treg/CD4+CD25+TE比率在感染后第3～14天显著降低(P<0.05);随后逐渐上升,在感染后第45天和第60天均显著高于对照组(P<0.05).结论 MCMV可在慢性感染期诱导CD4+CD25+Foxp3+Treg种群扩增,抑制CD4+CD25+Foxp3-Treg活化增殖,这可能是MCMV逃避特异性免疫而实现长期潜伏的机制之一.
목적 재정체수평탐토소서거세포병독(MCMV)감염대소서조절성T세포(Treg)아군CD4+CD25+Foxp3+T세포분화화CD4+CD25+Foxpp3-효응성T세포(TE)활화적영향.방법 건립전신파산형MCMV감염소서모형,의거주요장기내병독적도,학정감염후제28천위본모형급、만성기계정점.42지소서분별우감염MCMV후제1、3、7、14、28、45화60천처사6지;령설42지소서작위대조.류식세포술검측CD4+CD25+F0xp34+Treg화활화적CD4+CD25+Foxp3-TE재비단개핵세포중소점비솔변화.결과 MCMV감염급성기CD4+CD25+Foxp3+Treg비솔지속강저,제28천좌우강지최저치,여대조조비교차이유통계학의의(P<0.01);수후직선상승,제45천고우기선,제60천승지봉치,여대조조비교차이유통계학의의(P<0.05);CD4+CD25+Foxp3-TE재감염후제3～7천고우대조조(P<0.05),기후지속하강,재감염후제45천화제60천현저저우대조조수평(P<0.05).Treg/CD4+CD25+TE비솔재감염후제3～14천현저강저(P<0.05);수후축점상승,재감염후제45천화제60천균현저고우대조조(P<0.05).결론 MCMV가재만성감염기유도CD4+CD25+Foxp3+Treg충군확증,억제CD4+CD25+Foxp3-Treg활화증식,저가능시MCMV도피특이성면역이실현장기잠복적궤제지일.
Objective To investigate the influence of murine cytomegalovirus on the expansion of CD+CD25+ Foxp3+ regulatory T cell (Treg) and the activation of CD4+ CD25+Foxp3 - effector T cell (TE) in vivo. Methods Forty-two BALB/c mice were intraperitoneally inoculated with appropriate amount ofMCMV Smith strain for establishing the model of infection, another 42 mice served as mocked-infected con-trois. Day 28 post MCMV infection was determined to be the demarcation point of the acute and chronic in- fection based on the viral load of major visceral organs. On day 1,3, 7, 14, 28, 45 and 60 post infection, splenocytes were prepared by means of routine method. The proportions of CD4+CD25+ Foxp3+Treg and CD4+CD25+Foxp3- activated TE in T lymphocyte were measured by flow cytometry. Results The propor- tion of CD+CD25+ Foxp3+ T cells in T lymphocytes was persistently suppressed since day 7 post infection, and fell to the lowest level on day 28 post infection (P <0.01), then zoomed and reached the peak value on day 60 post infection (P < 0.05). CD4+CD25+ Foxp3 - TE proportion was up to the highest on day 3 post infection(P < 0. 01), then suppressed and in significantly lower level since day 45 post infection (P < 0.05). Treg/CD+TE ratio was in lower level on day 3 to 14 post infection(P <0.05) ; but on day 45 and day 60 post infection Treg/CD+ TE ratio was markedly increased (P < 0.05). Conclusion MCMV infec- tion can increase the CD+CD25+Foxp3+ Treg proportion, and inhibit CD4+T cells activation in chronic in- fection phase, which is likely to suppress the function of antiviral immunity in the infected host to cause a persistent latent infection.