CAJ | 학술논문

目的了解检测外周血液癌胚抗原mRNA(CEA mRNA)、细胞角蛋白19 mRNA(CK19mRNA)和端粒酶在监测肺癌血液转移中的应用价值.方法外周血液标本应用反转录-套式-聚合酶链反应检测103例肺癌患者(肺癌组)、57例非癌性肺部疾病患者(非肿瘤组)及36例健康志愿者(健康组)CEA mRNA和CK19 mRNA,端粒重复序列扩增-微孔板杂交-酶联免疫分析检测端粒酶.结果肺癌组血液三种肿瘤标志物的阳性率显著高于非肿瘤组和健康组(P<0.001),CEA mRNA和端粒酶的敏感度显著高于CK19 mRNA(P<0.01),TNM Ⅲ、Ⅳ期的阳性率显著高于Ⅰ期(P<0.05).结论检测血液CEA mRNA、CK19 mRNA和端粒酶有助于发现肺癌血液扩散,其中CEA mRNA和端粒酶的价值高于CK19 mRNA,联合检测可进一步提高敏感度.
목적 료해검측외주혈액암배항원mRNA(CEA mRNA)、세포각단백19 mRNA(CK19mRNA)화단립매재감측폐암혈액전이중적응용개치.방법 외주혈액표본응용반전록-투식-취합매련반응검측103례폐암환자(폐암조)、57례비암성폐부질병환자(비종류조)급36례건강지원자(건강조)CEA mRNA화CK19 mRNA,단립중복서렬확증-미공판잡교-매련면역분석검측단립매.결과 폐암조혈액삼충종류표지물적양성솔현저고우비종류조화건강조(P<0.001),CEA mRNA화단립매적민감도현저고우CK19 mRNA(P<0.01),TNM Ⅲ、Ⅳ기적양성솔현저고우Ⅰ기(P<0.05).결론 검측혈액CEA mRNA、CK19 mRNA화단립매유조우발현폐암혈액확산,기중CEA mRNA화단립매적개치고우CK19 mRNA,연합검측가진일보제고민감도.
Objective To understand the clinical value on the detection of carcinoembryonic antigen mRNA (CEA mRNA), cytokeratin 19 mRNA (CK19 mRNA) and telomerase in blood for the monitoring of blood metastasis of lung cancer. Methods CEA mRNA and CK19 mRNA were detected by reverse transcriptase-nested primers-polymerase chain reaction, telomerase was detected by telomeric repeat amplification protocol-hybridism-enzyme-linked immunosorbent assay in peripheral blood. Results The positive rates of the three tumor markers in lung cancer group were much higher than the non-tumor group and the health group (P<0.001). The sensibility of CEA mRNA and telomerase were much higher than CK19 mRNA (P<0.01). The positive rates of the markers in TNM stages Ⅲ and Ⅳ were much higher than in stages Ⅰ (P<0.05 to P<0.01). Conclusion It had high value that detecting CEA mRNA, CK19 mRNA and telomerase in peripheral blood on the discovery of blood metastasis of lung cancer. Among them, the clinical value of CEA mRNA and telomerase are higher than CK19 mRNA, and combined assay of the markers can improve the sensibility.