CAJ | 학술논문

目的研究昆明山海棠Tripterygium hypoglaucum(Celastraceae)(THH)根部水提液对早幼粒白血病HL-60细胞凋亡的诱导作用及机制.方法通过特征性的形态学观察,Annexin-V标记,以及流式细胞仪检测中次G1峰的形成确定THH的诱导细胞凋亡作用;应用Western Blotting研究THH对c-Mye蛋白表达的影响.结果THH根部水提液可诱导早幼粒白血病HL-60细胞凋亡.在浓度高于18μg/mL时,THH对HL-60细胞的诱导凋亡作用呈现浓度与时间的相关性.在THH处理2～4 h后,癌基因蛋白c-Myc表达降低99%以上.结论THH抑制了c-Myc蛋白的翻译或后翻译过程,从而降低了c-Myc在细胞周期运转中的作用,导致大量细胞凋亡.
목적연구곤명산해당Tripterygium hypoglaucum(Celastraceae)(THH)근부수제액대조유립백혈병HL-60세포조망적유도작용급궤제.방법통과특정성적형태학관찰,Annexin-V표기,이급류식세포의검측중차G1봉적형성학정THH적유도세포조망작용;응용Western Blotting연구THH대c-Mye단백표체적영향.결과THH근부수제액가유도조유립백혈병HL-60세포조망.재농도고우18μg/mL시,THH대HL-60세포적유도조망작용정현농도여시간적상관성.재THH처리2～4 h후,암기인단백c-Myc표체강저99%이상.결론THH억제료c-Myc단백적번역혹후번역과정,종이강저료c-Myc재세포주기운전중적작용,도치대량세포조망.
Object To investigate the effects of the root aqueous extract of Tripterygium hypoglaucum(Levl.) Hutch (Celastraceae) (THH) on human promyelocytic leukemia HL-60 cells and its mechanism.Methods Cell growth and toxicity assays, flow cytometry, confocal fluorescence microscopy and Annexin-V labeling, and Western Blotting Assay were used. Results THH causedmorphological changes hypodiploid sub-G1 cell accumulation and induced dose- and time-dependent apoptosis in HL-60 cells at concentrations above 18 μg/mL. Western Blotting analysis showed a more than 99% decrease of the nuclear oncoprotein c-Myc after 2-4 h of THH treatment. Conclusion THH decreases c-Myc protein expression,leading to the deactivation of cell cycle progression pathways and an accumulation of hypodiploid sub-G1cells thateventually enter apoptosis.