CAJ | 학술논문

目的研究重组人白介素-2吸入给药对小鼠克雷伯肺炎感染的保护作用.方法小鼠以滴鼻感染法进行感染.感染过程中,以鼻腔插管注入法和皮下注射给予小鼠重组人白介素-2.同时另设正常组、阴性对照组、模型组和阳性对照组(庆大霉素组).观察各组动物肺病理变化,比较平均死亡时间和一周内死亡率,测定支气管肺泡洗涤液中总蛋白、白蛋白含量,碱性磷酸酶、乳酸脱氢酶活力.结果重组人白介素-2给药组肺病理变化与模型组和阴性对照组相比显著减轻,支气管肺泡洗涤液中总蛋白、白蛋白含量和碱性磷酸酶活力降低.结论吸入重组人白介素-2缓解感染后肺部病理变化,为研发重组人白介素-2吸入剂提供了依据.
목적연구중조인백개소-2흡입급약대소서극뢰백폐염감염적보호작용.방법소서이적비감염법진행감염.감염과정중,이비강삽관주입법화피하주사급여소서중조인백개소-2.동시령설정상조、음성대조조、모형조화양성대조조(경대매소조).관찰각조동물폐병리변화,비교평균사망시간화일주내사망솔,측정지기관폐포세조액중총단백、백단백함량,감성린산매、유산탈경매활력.결과중조인백개소-2급약조폐병리변화여모형조화음성대조조상비현저감경,지기관폐포세조액중총단백、백단백함량화감성린산매활력강저.결론흡입중조인백개소-2완해감염후폐부병리변화,위연발중조인백개소-2흡입제제공료의거.
Aim To determine the protective effect of recombinant human interleukin-2 (rhIL-2) in inhalant form on experimental respiratory tract infection with Klebsiella pneumoniae in mice. Methods Mice were infected with the method of nasal intubation drip. During infection, mice were given rhIL-2 by sc injection and the method of nasal intubation drip. There were normal group, vehicle group, model group, rhIL-2 groups and gentamicin group. In the end, the pathological changes in the lung were observed. The survival time and the mortality within a week of each group were recorded. The total protein content, the albumin content, the activity of alkaline phosphatase and the activity of lactic dehydrogenase of broncho-alveolar lavage fluid (BALF) were dertermined and compared. Results Symptoms of Klebsiella pneumoniae were remarkably relieved because of rhIL-2 administration. The total protein content, the albumin content, the activity of alkaline phosphatase and the activity of lactic dehydrogenase of BALF were less than those in the vehicle group and the model group. Conclusion Inhalation of rhIL2 can alleviate the pathological changes in the lung after infection. At the same dose, it could be seen that the effect of rhIL-2 in inhalant form was better than that of the injection.