CAJ | 학술논문

目的通过体外实验探讨应用树突状细胞制备的肿瘤疫苗抗脑胶质瘤的作用机制.方法培养大鼠树突状细胞,冻融法制备胶质瘤抗原,以肿瘤抗原致敏树突状细胞而制备胶质瘤疫苗.将实验动物分为4组,分别将胶质瘤疫苗、树突状细胞、生理盐水注入正常大鼠体内,到达预定时间取出大鼠脾脏,四噻唑蓝(MTT)法检测大鼠淋巴细胞活性.结果一次输入胶质瘤疫苗的正常大鼠的淋巴细胞对肿瘤细胞的杀伤活性明显低于3次输入胶质瘤疫苗的正常大鼠;比较输入培养8 d的树突状细胞和输入生理盐水的大鼠,其淋巴细胞对肿瘤细胞的杀伤活性无统计学差异.结论应用树突状细胞制备胶质瘤疫苗可明显激活体内抗肿瘤免疫反应.
목적통과체외실험탐토응용수돌상세포제비적종류역묘항뇌효질류적작용궤제.방법배양대서수돌상세포,동융법제비효질류항원,이종류항원치민수돌상세포이제비효질류역묘.장실험동물분위4조,분별장효질류역묘、수돌상세포、생리염수주입정상대서체내,도체예정시간취출대서비장,사새서람(MTT)법검측대서림파세포활성.결과일차수입효질류역묘적정상대서적림파세포대종류세포적살상활성명현저우3차수입효질류역묘적정상대서;비교수입배양8 d적수돌상세포화수입생리염수적대서,기림파세포대종류세포적살상활성무통계학차이.결론응용수돌상세포제비효질류역묘가명현격활체내항종류면역반응.
Objective To discuss the mechanism of the anti-glioma activity of the tumor vaccine made from the dendritic cell in vitro.Methods Culture dendritic cells of the rat, extract the glioma antigen by freeze-melt method and then get the vaccine by pulsing the denderitic cells with the glioma antigen. The experiment rats were divided into 4 groups. The vaccine, dendritic cells and 0.9% (g/ml) chlorine sodium saline were perfused to the rats. At the prearranged time, the spleen of the rats was taken out and was examined the anti-tumor activity of the T lymphatic cell of the spleen by methyl thiazolyl tetrazolium (MTT) method.Results The anti-tumor activity of the lymphocyte of the rats which was injected with glioma vaccine once was lower than that of the rats which were injected for 3 times. There was no statistic difference between the kill activity to the glioma cells of the lymphatic cells of the rats injected with dendritic cells cultured 8 days and the lymphatic cells of the rats injected with 0.9% (g/ml) chlorine sodium saline.Conclusion Glioma vaccine made from dendritic cells can stimulate the immune activity to kill the glioma cells obviously.