CAJ | 학술논문

目的采用两种技术检测糖尿病并发感染患者单核细胞趋化蛋白(MCP-1)mRNA与蛋白表达的变化.方法随机选择初诊糖尿病并发感染患者,应用酶联免疫吸附试验(ELISA)、竞争法逆转录聚合酶链反应(RT-PCR)及实时荧光定量PCR(FQ-PCR)检测患者感染治愈前、后MCP-1蛋白及mRNA定量水平;同时还分析了与其他参数的关系.结果糖尿病并发感染治疗前后趋化因子MCP-1 mRNA与蛋白表达均明显升高,MCP-1 mRNA(FQ-PCR)与蛋白表达水平呈正相关性,mRNA(竞争性密度比法)与其他结果未见明显相关性.结论利用FQ-PCR技术检测糖尿病并发感染患者MCP-1 mRNA与蛋白水平具有较高的一致性,对研究其病理机制和临床观察有重要意义.
목적 채용량충기술검측당뇨병병발감염환자단핵세포추화단백(MCP-1)mRNA여단백표체적변화.방법 수궤선택초진당뇨병병발감염환자,응용매련면역흡부시험(ELISA)、경쟁법역전록취합매련반응(RT-PCR)급실시형광정량PCR(FQ-PCR)검측환자감염치유전、후MCP-1단백급mRNA정량수평;동시환분석료여기타삼수적관계.결과 당뇨병병발감염치료전후추화인자MCP-1 mRNA여단백표체균명현승고,MCP-1 mRNA(FQ-PCR)여단백표체수평정정상관성,mRNA(경쟁성밀도비법)여기타결과미견명현상관성.결론 이용FQ-PCR기술검측당뇨병병발감염환자MCP-1 mRNA여단백수평구유교고적일치성,대연구기병리궤제화림상관찰유중요의의.
Objective To detect the expression of monoeyte ehemoattractant protein-1(MCP-1)mRNA and protein in patients with diabetes mellitus(DM)complicated with infeetion with two RTPCR techniques.Methods 48 patients with preliminary diagnosed DM complicated with infection were enrolled in the study.Expression of MCp-1 mRNA and protein were detected with enzyme-linked immunosorbent assay(ELISA),competitive polymerase chain reaction(RT-PCR),and real time fluoreseenee quatitative PCR(FQ-PCR)before and after infection curing.The relationship between MCP1 and other parameters was analyzed.Results The expression of mRNA and protein of MCP-1 were inereased in the DM patients complicated with infection before and after treatment.There is a positive correlation in expression level between MCP-1 mRNA(measured by FQ-PCR)and protein;but there was no correlation between MCP-1 and other parameters.Conclusion High concordance was presented in MCP-1 mRNA and protein expression detected with FQ-PCR assay.which may play a key role in elucidation of pathomechanism and clinical observation during DM complicated with infection.