CAJ | 학술논문

建立了虾青素手性异构体分离和检测的HPLC方法.条件:采用手性固定相色谱柱;Pirkle L-亮胺酸(4.6mm × 25cm,5μm);流动相:正己烷-四氧吠喃-异丙醇-三乙胺(77:17:3:3,V/V);紫外检测波长:474nm;流速:0.75ml/min.本法可应用于快速鉴别养殖三文鱼是否使用了人工合成虾青素,并可以初步判断是否为野生三文鱼.
건립료하청소수성이구체분리화검측적HPLC방법.조건:채용수성고정상색보주;Pirkle L-량알산(4.6mm × 25cm,5μm);류동상:정기완-사양폐남-이병순-삼을알(77:17:3:3,V/V);자외검측파장:474nm;류속:0.75ml/min.본법가응용우쾌속감별양식삼문어시부사용료인공합성하청소,병가이초보판단시부위야생삼문어.
A high performance liquid chromatography (HPLC) method was developed to identify salmon aquacultured with synthetic astaxanthin through determining relative ratio of chiral isomers of astaxanthin in salmon. Astaxanthin was extracted from flesh of salmon, passed through a Sep-Pak silica gel column for purification, and then analyzed by HPLC. The chromato-graphic conditions were Pirkle covalent L-leucine column (4.6 mm × 25 cm, 5μm) as the stationary phase, hexane-tetrahydro-furan-2-propanol-triethylamine (77:17:3:3, V/V) as mobile phase with flow rate of 0.75 ml/min, and detection wavelength at 474 nm. Distribution of chiral isomers of astaxanthin in wild salmon and salmon fed with natural feedstuff was similar. However, it revealed a significant difference in salmon fed with synthetic astaxanthin.