CAJ | 학술논문

背景:体外构建组织工程化心肌是当今医学领域的研究热点,支架材料的选择和设计是心肌组织工程的关键环节,但目前仍未找到理想的心肌支架材料.目的:对新型可降解材料聚氨酯进行体外评价,初步探讨其作为心肌组织工程支架的可行性.方法:以赖氨酸基二异氰酸酯为硬段,以赖氨酸为扩展链合成新型聚氨酯(PU-Lys).在拉力机上测试材料的缝合强度和拉伸强度;在37℃,pH=7.4的磷酸盐缓冲溶液中检测聚氨酯的降解性能;采用细胞培养MTT法、细胞形态学观察方法,分析该聚氨酯的细胞毒性.结果与结论:力学性能检测得出该聚氨酯的拉伸强度为(8.1±0.1)MPa,缝合强度为(12.2±0.8)N;体外降解8周后质量损失为(13.1±0.3)%;MTT比色法结果显示细胞毒性为0～1级;细胞形态学观察显示L929细胞在聚氨酯材料浸提液中呈梭形或三角形,贴壁良好.提示此种新型解聚氨酯具有良好的力学性能和降解性,细胞相容性良好,符合组织工程心肌支架材料的应用要求.
배경:체외구건조직공정화심기시당금의학영역적연구열점,지가재료적선택화설계시심기조직공정적관건배절,단목전잉미조도이상적심기지가재료.목적:대신형가강해재료취안지진행체외평개,초보탐토기작위심기조직공정지가적가행성.방법:이뢰안산기이이청산지위경단,이뢰안산위확전련합성신형취안지(PU-Lys).재랍력궤상측시재료적봉합강도화랍신강도;재37℃,pH=7.4적린산염완충용액중검측취안지적강해성능;채용세포배양MTT법、세포형태학관찰방법,분석해취안지적세포독성.결과여결론:역학성능검측득출해취안지적랍신강도위(8.1±0.1)MPa,봉합강도위(12.2±0.8)N;체외강해8주후질량손실위(13.1±0.3)%;MTT비색법결과현시세포독성위0～1급;세포형태학관찰현시L929세포재취안지재료침제액중정사형혹삼각형,첩벽량호.제시차충신형해취안지구유량호적역학성능화강해성,세포상용성량호,부합조직공정심기지가재료적응용요구.
BACKGROUND: In vitro construction of tissue engineered cardiac muscle becomes a hot spot in recent years, and the selection and design of scaffold is the key link. However, there is lack of ideal cardiac tissue engineering scaffold material. OBJECTIVE: To evaluate the novel biodegradable polyurethane in vitro, and to discuss the feasibility of polyurethane as cardiac tissue engineeru scaffold. METHODS: A new type polyurethane (PV-Lys) was synthesized using diphenylmethane-4,4'-diisocyanate as hard segment and lysine as expand chain. The tensile and suture strength were tested in vitro respectively, hydrolytic degradation was carded out in phosphate buffer saline of pH 7.4 at 37 ℃, and cytotoxicity was evaluated by MTT measurement and morphological observation. RESULTS AND CONCLUSION: The tensile strength of the polyurethane was up to (8.1±0.1) MPa, and the suture strength was (12.2+0.8) N. The average value of the mass loss of PV-Lys was (13.1+0.3)% at 8 weeks of in vitro hydrolytic degradation. MTT assay results showed that the cytotoxic grade of the novel PV-Lys was 0-1. Cell morphology observation showed that the L929 cells were spindle-shaped or tdangular with good stretch. This PV-Lys scaffold is with favorable mechanical property, cytocompatibility, biodegradable property, which meets the requirements of tissue engineering application.