中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2012年
1期
20-22
,共3页
邓磊%唐源%林静%陆小军%薛建新%王立帅%周麟%邹艳%应斌武%李甘地%卢铀
鄧磊%唐源%林靜%陸小軍%薛建新%王立帥%週麟%鄒豔%應斌武%李甘地%盧鈾
산뢰%당원%림정%륙소군%설건신%왕립수%주린%추염%응빈무%리감지%로유
癌,非小细胞肺%受体,表皮生长因子%聚合酶链反应
癌,非小細胞肺%受體,錶皮生長因子%聚閤酶鏈反應
암,비소세포폐%수체,표피생장인자%취합매련반응
Carcinoma,non-small-cell lung%Receptor,epidermal growth factor%Polymerase chain reaction
目的 分析比较等位基因特异性寡核苷酸聚合酶链反应( ASO -PCR)和双环探针特异引物荧光PCR (BPSP-qPCR)的表皮生长因子受体(EGFR)基因突变检测敏感性.方法 收集2009年9月至2010年12月在四川大学华西医院病理科留存非小细胞肺癌标本96例,行ASO-PCR检测EGFR突变,其中39例再行BPSP-qPCR检测.结果 ASO-PCR测得突变率为30.2％ (29/96).其中女性高于男性[45.5％ (20/44)比17.3％ (9/52),P=0.003],不吸烟者高于吸烟者[44.1％ (26/59)比8.1％ (3/37),P＜0.001],腺癌高于非腺癌[37.0％ (27/73)比8.7％ (2/23),P=0.01].ASO-PCR检测阴性标本中,具有腺癌和不吸烟特征,BPSP-qPCR检测19-del和L.858R阳性的检出率可提高10.3％ (4/39),BPSP-qPCR检测阳性率明显高于ASO-PCR [ 66.7％( 26/39)比41.0％( 16/39),P=0.02].结论 BPSP-qPCR比ASO-PCR检测EGFR敏感基因突变有更高的敏感性.
目的 分析比較等位基因特異性寡覈苷痠聚閤酶鏈反應( ASO -PCR)和雙環探針特異引物熒光PCR (BPSP-qPCR)的錶皮生長因子受體(EGFR)基因突變檢測敏感性.方法 收集2009年9月至2010年12月在四川大學華西醫院病理科留存非小細胞肺癌標本96例,行ASO-PCR檢測EGFR突變,其中39例再行BPSP-qPCR檢測.結果 ASO-PCR測得突變率為30.2％ (29/96).其中女性高于男性[45.5％ (20/44)比17.3％ (9/52),P=0.003],不吸煙者高于吸煙者[44.1％ (26/59)比8.1％ (3/37),P＜0.001],腺癌高于非腺癌[37.0％ (27/73)比8.7％ (2/23),P=0.01].ASO-PCR檢測陰性標本中,具有腺癌和不吸煙特徵,BPSP-qPCR檢測19-del和L.858R暘性的檢齣率可提高10.3％ (4/39),BPSP-qPCR檢測暘性率明顯高于ASO-PCR [ 66.7％( 26/39)比41.0％( 16/39),P=0.02].結論 BPSP-qPCR比ASO-PCR檢測EGFR敏感基因突變有更高的敏感性.
목적 분석비교등위기인특이성과핵감산취합매련반응( ASO -PCR)화쌍배탐침특이인물형광PCR (BPSP-qPCR)적표피생장인자수체(EGFR)기인돌변검측민감성.방법 수집2009년9월지2010년12월재사천대학화서의원병이과류존비소세포폐암표본96례,행ASO-PCR검측EGFR돌변,기중39례재행BPSP-qPCR검측.결과 ASO-PCR측득돌변솔위30.2％ (29/96).기중녀성고우남성[45.5％ (20/44)비17.3％ (9/52),P=0.003],불흡연자고우흡연자[44.1％ (26/59)비8.1％ (3/37),P＜0.001],선암고우비선암[37.0％ (27/73)비8.7％ (2/23),P=0.01].ASO-PCR검측음성표본중,구유선암화불흡연특정,BPSP-qPCR검측19-del화L.858R양성적검출솔가제고10.3％ (4/39),BPSP-qPCR검측양성솔명현고우ASO-PCR [ 66.7％( 26/39)비41.0％( 16/39),P=0.02].결론 BPSP-qPCR비ASO-PCR검측EGFR민감기인돌변유경고적민감성.
Objective To compare the detection sensitivity of epidermal growth factor receptor (EGFR) mutations between allele specific oligonucleotide PCR(ASO-PCR) and bi-loop probe and specific primer quantitative PCR (BPSP-qPCR).Methods A total of 96 non-small cell lung cancer specimens were selected from West China Hospital from September 2009 to December 2010.ASO-PCR was developed to detect the presence of classical EGFR mutations.A total 39 available specimens were also tested by BPSP- qPCR.Results EGFR mutation detection rate was 30.2％ (26/96) by ASO-PCR.The mutation rate was higher in female than in male patients[45.5％ (20/44) vs.17.3％ (9/52),P =0.003],non-smokers than smokers[ 44.1％ (26/59) vs.8.1％ (3/37),P ＜ 0.001 ] and adenocarcinomas than other subtypes of lung cancer [ 37.0％ ( 27/73 ) vs.8.7％ (2/23),P =0.01 ].Among mutation negative cases by ASO-PCR,BPSP-qPCR increased the rate of detection of 19-del and L858R mutation by 10.3％ (4/39) inadenocarcinomas and non-smoking subset.Overall,the mutation detection rate of BPSP-qPCR was higher than that of ASO-PCR [66.7％ (26/39) vs.41.0％ (16/39),P=0.02].Conclusion BPSP-qPCR has a better detection sensitivity than that of ASO-PCR.
