中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2008年
8期
1066-1068
,共3页
白细胞介素2%肝炎,乙型,慢性%树突细胞
白細胞介素2%肝炎,乙型,慢性%樹突細胞
백세포개소2%간염,을형,만성%수돌세포
Interleukin-2%Hepatitis B,chronic%Dendritic cells
目的 通过白介素-2刺激体外培养的慢性乙肝患者外周血树突状细胞,检测树突状细胞白介素-2的表达和培养液中IL-12的浓度,以探讨白介素-2对慢性乙肝患者外周血树突状细胞(DC)的影响,进而探讨白介素-2治疗慢性乙肝的作用机理.方法 抽取慢性乙肝患者外周血分离培养树突状细胞,对照组加入GM-CSF和IL-4,试验组加入GM-CSF、IL-4和白介素-2,培养第9天时收集细胞,应用流式细胞仪检测CD,第12天时收集上清,用ELISA法检测IL-12的浓度.结果 应用白介素-2的试验组树突状细胞CD40、CD86、CD80、CD83的表达有所增加,上清中IL-12的浓度也较高.结论 白介素-2促进慢性乙肝患者外周树突状细胞表型成熟和功能增强.
目的 通過白介素-2刺激體外培養的慢性乙肝患者外週血樹突狀細胞,檢測樹突狀細胞白介素-2的錶達和培養液中IL-12的濃度,以探討白介素-2對慢性乙肝患者外週血樹突狀細胞(DC)的影響,進而探討白介素-2治療慢性乙肝的作用機理.方法 抽取慢性乙肝患者外週血分離培養樹突狀細胞,對照組加入GM-CSF和IL-4,試驗組加入GM-CSF、IL-4和白介素-2,培養第9天時收集細胞,應用流式細胞儀檢測CD,第12天時收集上清,用ELISA法檢測IL-12的濃度.結果 應用白介素-2的試驗組樹突狀細胞CD40、CD86、CD80、CD83的錶達有所增加,上清中IL-12的濃度也較高.結論 白介素-2促進慢性乙肝患者外週樹突狀細胞錶型成熟和功能增彊.
목적 통과백개소-2자격체외배양적만성을간환자외주혈수돌상세포,검측수돌상세포백개소-2적표체화배양액중IL-12적농도,이탐토백개소-2대만성을간환자외주혈수돌상세포(DC)적영향,진이탐토백개소-2치료만성을간적작용궤리.방법 추취만성을간환자외주혈분리배양수돌상세포,대조조가입GM-CSF화IL-4,시험조가입GM-CSF、IL-4화백개소-2,배양제9천시수집세포,응용류식세포의검측CD,제12천시수집상청,용ELISA법검측IL-12적농도.결과 응용백개소-2적시험조수돌상세포CD40、CD86、CD80、CD83적표체유소증가,상청중IL-12적농도야교고.결론 백개소-2촉진만성을간환자외주수돌상세포표형성숙화공능증강.
Objective To evaluate the role of IL-2 dendritic cell in chronic hepatitis B,peri-dendritic cells were cultured,cytokins CD4O,CD86,CD80,CD83 and IL-2 were measured by ELLISA.Methods 20 chronic hepatitis B patients were selected,each one donated 15ml blood.dentritic cells were cultured,and IL-4 1000u/ml rhGM-CSF 1000u/ml was given each well,while IL-2 100u/ml was given each control well.CD40,CD86,CD80 and CD83 were measured by FLSC at 9th day.IL-2 was detected by ELISA at 12th day.Results The level of CD40,CD86,CD80 and CD83 in increased when using IL-2,and dentritic cell secreted IL-2 in chronic hepatitis.Conclusion The results suggest that IL-2 can promote the phenotypic and functional maturation of PC.
