CAJ | 학술논문

目的探索非酒精性单纯性脂肪肝(NAFL)患者外周血CD4+ CD25+调节性T细胞(Treg)数量变化与临床意义.方法流式细胞术检测50例NAFL患者以及50例健康对照者外周血CD4+ CD25+ Treg的变化,采用成组t检验或Mann-Whitney U检验及Spearman秩相关检验进行统计学分析.结果 NAFL患者外周血CD4+ CD25+ Treg占CD4+T淋巴细胞的比例为(5.39±1.94)％,高于健康对照者的(4.21±1.52)％,差异有统计学意义(t=3.385,P＜0.01).Treg数量与三酰甘油(TG)、体质指数(BMI)水平呈正相关(r=0.307、0.251,P=0.002、0.012),与高密度脂蛋白胆固醇(HDL-C)呈负相关(r=-0.306,P=0.002).Treg在高BMI组、高TG组、低HDL-C 组、高血压组及代谢综合征(MS)组升高(t=2.294、2.533、3.154、2.010、4.454,均P＜0.05),在高空腹血糖组差异无统计学意义(U=1143.500,P=0.471).结论 NAFL患者外周血Treg数量增加,可能是NAFL作为MS组分之一,与MS时机体处于慢性低度炎性反应状态所表现出的促炎与抗炎平衡紊乱有关.
목적 탐색비주정성단순성지방간(NAFL)환자외주혈CD4+ CD25+조절성T세포(Treg)수량변화여림상의의.방법 류식세포술검측50례NAFL환자이급50례건강대조자외주혈CD4+ CD25+ Treg적변화,채용성조t검험혹Mann-Whitney U검험급Spearman질상관검험진행통계학분석.결과 NAFL환자외주혈CD4+ CD25+ Treg점CD4+T림파세포적비례위(5.39±1.94)％,고우건강대조자적(4.21±1.52)％,차이유통계학의의(t=3.385,P＜0.01).Treg수량여삼선감유(TG)、체질지수(BMI)수평정정상관(r=0.307、0.251,P=0.002、0.012),여고밀도지단백담고순(HDL-C)정부상관(r=-0.306,P=0.002).Treg재고BMI조、고TG조、저HDL-C 조、고혈압조급대사종합정(MS)조승고(t=2.294、2.533、3.154、2.010、4.454,균P＜0.05),재고공복혈당조차이무통계학의의(U=1143.500,P=0.471).결론 NAFL환자외주혈Treg수량증가,가능시NAFL작위MS조분지일,여MS시궤체처우만성저도염성반응상태소표현출적촉염여항염평형문란유관.
Objective To investigate the changes and clinical significance of the frequency of circulating CD4+ CD25+ regulatory T cells (Treg) in nonalcoholic fatty liver (NAFL) patients.Methods CD4+ CD25+ Treg in the peripheral blood from 50 NAFL patients and 50 healthy subjects were quantitatively analyzed using flow cytometry. Group t test or Mann-Whitney U test and Spearman's rank correlation test were used for statistical analysis.Results The proportion of circulating CD4+CD25+ Treg in NAFL patients was (5.39 ± 1.94)％,which was significantly higher than that in healthy controls [(4.21±1.52)％](t=3.385,P＜0.01).Further analysis revealed that the frequency of Treg was positively correlated with triglyceride (TG) level and body mass index (r=0.307 and 0.251,respectively; P=0.002 and 0.012,respectively),and negatively correlated with high density lipoproteincholesterol (HDL-C) (r=-0.306,P=0.002).Meanwhile,Treg in patients with high body mass index,high TG,low HDL-C,hypertension and metabolic syndrome (MS) were all higher than those in controls (t=2.294,2.533,3.154,2.010 and 4.454,respectively; all P＜0.05).But there was no significant difference between patients with high fasting blood glucose and controls (U=1143.500,P=0.471).Conclusion The increased frequency of peripheral Treg in NAFL patients may have some relations with the imbalance of proinflammation and anti-inflammation in NAFL patients who coexisting with MS.