中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2010年
4期
242-245
,共4页
张晓君%傅志仁%康印东%宋少华%沈筱芸%洪善娟%丁国善%朱有华%王正昕
張曉君%傅誌仁%康印東%宋少華%瀋篠蕓%洪善娟%丁國善%硃有華%王正昕
장효군%부지인%강인동%송소화%침소예%홍선연%정국선%주유화%왕정흔
他克莫司%T_H17细胞%细胞分化%细胞增殖
他剋莫司%T_H17細胞%細胞分化%細胞增殖
타극막사%T_H17세포%세포분화%세포증식
Tacrolimus%T_H 17 cells%Cell differentiation%Cell proliferation
目的 观察在T_H17型细胞极化环境下,他克莫司(Tac)对小鼠T_H17型细胞分化增殖的影响及机制.方法 将小鼠脾脏初始CD4+CD25+T淋巴细胞使用抗CD3抗体及抗CD28抗体进行活化,使用转化生长因子β_1,(TGF-β_1,)和白细胞介素6(IL-6)等细胞因子进行诱导,促使其向T_H17型细胞方向分化.将体外培养的T_H 17型细胞分为4组,分别用不同剂量的Tac进行干预,即:Tac0 ng/ml组(对照组)、Tac 0.1 ng/ml组、Tac 1 ng/ml组和Tae 10 ng/ml组.应用流式细胞术检测各组T_H 17型细胞亚群纯度,使用逆转录聚合酶链反应(RT.PCR)检测各组IL-17 mRNA相对表达量.结果 Tac可抑制小鼠T_H17型细胞亚群的分化增殖,降低IL-17 mRNA的表达量,且呈剂量依赖性,各组间比较,差异均有统计学意义(P<0.05).结论 Tac通过抑制T_H17型细胞胞浆内的钙调磷酸酶,抑制T_H17型细胞亚群的分化增殖,抑制IL-17 mRNA产生并减轻炎症进程,从而抑制排斥反应.
目的 觀察在T_H17型細胞極化環境下,他剋莫司(Tac)對小鼠T_H17型細胞分化增殖的影響及機製.方法 將小鼠脾髒初始CD4+CD25+T淋巴細胞使用抗CD3抗體及抗CD28抗體進行活化,使用轉化生長因子β_1,(TGF-β_1,)和白細胞介素6(IL-6)等細胞因子進行誘導,促使其嚮T_H17型細胞方嚮分化.將體外培養的T_H 17型細胞分為4組,分彆用不同劑量的Tac進行榦預,即:Tac0 ng/ml組(對照組)、Tac 0.1 ng/ml組、Tac 1 ng/ml組和Tae 10 ng/ml組.應用流式細胞術檢測各組T_H 17型細胞亞群純度,使用逆轉錄聚閤酶鏈反應(RT.PCR)檢測各組IL-17 mRNA相對錶達量.結果 Tac可抑製小鼠T_H17型細胞亞群的分化增殖,降低IL-17 mRNA的錶達量,且呈劑量依賴性,各組間比較,差異均有統計學意義(P<0.05).結論 Tac通過抑製T_H17型細胞胞漿內的鈣調燐痠酶,抑製T_H17型細胞亞群的分化增殖,抑製IL-17 mRNA產生併減輕炎癥進程,從而抑製排斥反應.
목적 관찰재T_H17형세포겁화배경하,타극막사(Tac)대소서T_H17형세포분화증식적영향급궤제.방법 장소서비장초시CD4+CD25+T림파세포사용항CD3항체급항CD28항체진행활화,사용전화생장인자β_1,(TGF-β_1,)화백세포개소6(IL-6)등세포인자진행유도,촉사기향T_H17형세포방향분화.장체외배양적T_H 17형세포분위4조,분별용불동제량적Tac진행간예,즉:Tac0 ng/ml조(대조조)、Tac 0.1 ng/ml조、Tac 1 ng/ml조화Tae 10 ng/ml조.응용류식세포술검측각조T_H 17형세포아군순도,사용역전록취합매련반응(RT.PCR)검측각조IL-17 mRNA상대표체량.결과 Tac가억제소서T_H17형세포아군적분화증식,강저IL-17 mRNA적표체량,차정제량의뢰성,각조간비교,차이균유통계학의의(P<0.05).결론 Tac통과억제T_H17형세포포장내적개조린산매,억제T_H17형세포아군적분화증식,억제IL-17 mRNA산생병감경염증진정,종이억제배척반응.
Objective To explore the effects of tacrolimus (Tac) on the differentiation and proliferation of T_H 17 (T helper type 17) cells in mice under T_H 17 polarized conditions and the mechanism.Methods Murine naive CD4+ CD25- T cells were stimulated with plate-bound anti-CD3 and anti-CD28, and induced by the cytokines such as TGF-β1 and IL-6, etc.The in vitro T_H17 cells in mice were divided into 4 groups: Tac 0 ng/ml group (control group), Tac 0.1 ng/ml group, Tac 1 ng/ml group and Tac 10 ng/ml group.Tac was added to the cells at the desired time point and desired concentrations.The subsets of CD4+ T_H 17 cells were examined by flow cytometry.The expression of IL-17 mRNA was assayed by RT-PCR Results Tac inhibited the differentiation and proliferation of T_H 17 cells in mice in a dose-dependent manner.The expression of IL-17 mRNA was inhibited either.There were significant difference among the groups (P< 0.05 ).Conclusion By suppression the activation of calcineurin in T_H 17 cells, Tac inhibits the differentiation and proliferation of T_H 17 cells, reduces the expression of IL-17 mRNA and alleviates the process of inflammation, thereby suppressing rejection.
