CAJ | 학술논문

目的观察在组织工程骨内植入血管、神经束对大段组织工程骨成骨的]响,并探讨神经肽类物质在其中的分布.方法 36只新西兰大白兔,随机分为3组:A组,单纯组织工程骨组;B组,血管束植入组(股血管束);C组,感觉神经束植入组(隐神经柬).每只兔均在右侧股骨制造长1.5 cm的段缺性骨与骨膜缺损,钢板固定后植入3种方法制备的组织工程骨.术后4、8、12周行大体观察、X线观察、新生骨组织定量观察成骨效果,神经肽Y(NPY)及降钙素基因相关肽(CGRP)的免疫组织化学染色观察其在组织工程骨内的分布,并行半定量分析.结果在组织工程骨内植入血管、神经后,比单纯组织工程骨的修复效果有提高,术后12周新生骨小梁占骨缺损面积的平均百分数分别为(48.67±4.21)%、(75.13±5.75)%、(73.44±2.99)%.对新生骨的组织学染色半定量分析显示3组间差异有统计学意义(F=105.735,P＜0.01),免疫组织化学染色显示神经肽类物质NPY、CGRP在B组与C组中的表达高于A组,差异有统计学意义(F=30.509及16.475,P＜0.01).结论在组织工程骨内植人血管、神经束在早期可促进成骨,其机制可能是植入的血管、神经束在组织工程骨内发芽并分泌神经肽所致.
목적 관찰재조직공정골내식입혈관、신경속대대단조직공정골성골적]향,병탐토신경태류물질재기중적분포.방법 36지신서란대백토,수궤분위3조:A조,단순조직공정골조;B조,혈관속식입조(고혈관속);C조,감각신경속식입조(은신경간).매지토균재우측고골제조장1.5 cm적단결성골여골막결손,강판고정후식입3충방법제비적조직공정골.술후4、8、12주행대체관찰、X선관찰、신생골조직정량관찰성골효과,신경태Y(NPY)급강개소기인상관태(CGRP)적면역조직화학염색관찰기재조직공정골내적분포,병행반정량분석.결과 재조직공정골내식입혈관、신경후,비단순조직공정골적수복효과유제고,술후12주신생골소량점골결손면적적평균백분수분별위(48.67±4.21)%、(75.13±5.75)%、(73.44±2.99)%.대신생골적조직학염색반정량분석현시3조간차이유통계학의의(F=105.735,P＜0.01),면역조직화학염색현시신경태류물질NPY、CGRP재B조여C조중적표체고우A조,차이유통계학의의(F=30.509급16.475,P＜0.01).결론 재조직공정골내식인혈관、신경속재조기가촉진성골,기궤제가능시식입적혈관、신경속재조직공정골내발아병분비신경태소치.
Objective To investigate the osteogenesis effects in vivo on the construction of vascularization and neurotization of large tissue-engineered bone and approach the distribution of neuropeptide initially. Methods Thirty-six New Zeland rabbits were divided into 3 groups randomly and equally: tissueengineering group ( A), vascular bundle group ( B, femoral vascular bundle), sensory nerve tract group ( C, saphenous nerves). A segmental bone defects of 1.5 cm long were made at the right femur in each animal. After plate fixation, the defect was implanted respectively with the engineered bone prepared by the 3methods mentioned above. Four, 8 and 12 weeks postoperatively, gross observation, X-ray observation and HE staining were adopted to evaluate the engineered bone and the new bone formation was measured by image analysis. The distribution of neuropeptide Y (NPY) and calcitonin gene related peptide (CGRP) in the new bone was detected by immunohistochemistryand analyzed semiquantitativly. Results After implantation of vascular bundle and sensory nerve into the tissue-engineered bone, the reparative effect was obviously better than the control group. New bone formation was gradually increased ( F = 105. 735, P ＜0. 01 ). The percentage of newly formed trabeculae in bone defect area at the 12th week was (48.67 ±4.21 ) %, (75.13 ± 5.75 ) %, (73.44 ± 2. 99) % in groups A, B and C, respectively. The effect of sensory nerve tract or vascular bundle implantation in osteogenesis have statistical significance ( F = 290. 008,P＜0. 01 ). The factorial design ANOVA showed time, sensory nerve tract and vascular bundle implantation had interaction (F=3. 177, P ＜ 0. 05 ). The formation of new bone in group B was increased as compared with that in group A and group C at the 4th week, that in group C was increased as compared with that in group A, and that in group B and group C was increased as compared with that in group A at 8th and 12th week. No obvious difference was found between group C and group B at the 8th and 12th week,The expression levels of NPY and CGRP in group B and group C were obviously higher than in group A at the three time points ( F = 30. 509 and 16. 475, P ＜ 0. 01 ). Conclusion Implantation of sensory nerve tract or vascular bundle into tissue-engineered bone can improve osteogenesis. Sprouting and secretion of neuropeptide by the implanted sensory nerve or vascular bundle may be the two possible mechanisms.