CAJ | 학술논문

目的探讨人血清中P53抗体与广西肝癌家族聚集性的相关性.方法应用酶联免疫吸附法定量检测广西肝癌高发区164对肝癌高发家族成员和无癌家族成员血清中P53抗体水平,并应用单因素方法分析血清P53抗体水平与广西肝癌家族聚集性的相关性.结果肝癌高发家族成员血清P53抗体水平高于无癌家族成员(Z=-3.04,P=0.002).排除乙型肝炎病毒感染因素的影响后,这一趋势仍然存在(P=0.011).在肝癌高发家族中,不同亲缘关系的成员之间血清P53抗体水平的差异有统计学意义(x2=11.593,P=0.021),并具有随亲缘系数下降而下降的趋势.肝癌高发家族中P53抗体高表达率(95/164)要高于无癌家族成员(71/164)(P=0.006).随着患病人数的增加,肝癌高发家族中P53抗体高表达率也相应上升(x2=16.068,P=0.000).同胞患病家族中P53高表达率要高于父母患病家族(x2=12.679 P=0.002).单因素分析结果提示,P53抗体高表达是肝癌家族聚集性的风险因素(OR=2.087,95％CI:1.270～3.431).结论血清中P53抗体高表达水平可能是广西肝癌家族聚集性的风险因素.
목적 탐토인혈청중P53항체여엄서간암가족취집성적상관성.방법 응용매련면역흡부법정량검측엄서간암고발구164대간암고발가족성원화무암가족성원혈청중P53항체수평,병응용단인소방법분석혈청P53항체수평여엄서간암가족취집성적상관성.결과 간암고발가족성원혈청P53항체수평고우무암가족성원(Z=-3.04,P=0.002).배제을형간염병독감염인소적영향후,저일추세잉연존재(P=0.011).재간암고발가족중,불동친연관계적성원지간혈청P53항체수평적차이유통계학의의(x2=11.593,P=0.021),병구유수친연계수하강이하강적추세.간암고발가족중P53항체고표체솔(95/164)요고우무암가족성원(71/164)(P=0.006).수착환병인수적증가,간암고발가족중P53항체고표체솔야상응상승(x2=16.068,P=0.000).동포환병가족중P53고표체솔요고우부모환병가족(x2=12.679 P=0.002).단인소분석결과제시,P53항체고표체시간암가족취집성적풍험인소(OR=2.087,95％CI:1.270～3.431).결론 혈청중P53항체고표체수평가능시엄서간암가족취집성적풍험인소.
Objective To assess the correlation between familial clustering of hepatocellular carcinoma (HCC) and the level of anti-P53 in human serum in Guangxi.Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect anti-P53 in 164 members from 20 HCC families and 164 members from non-cancer control families.Univariate analysis was performed to assess the correlation between seral level of P53 antibody and familial clustering of HCC.Results The level of P53 antibody was significantly higher in the members of HCC families than controls (Z=-3.04,P=0.002).After eliminating the interference of hepatitis B virus infection,this tendency still remains (P=0.011).And there was a significant difference between relatives of different degrees from HCC families (x2=11.593,P=0.021),with the expression of anti-P53 declining along with decrease in relationship coefficient.Furthermore,the number of individuals with high anti-P53 expression was also significantly greater in HCC families (95/164) than controls (71/164) (P=0.006).And the expression was rising along with the increasing HCC numbers (x2 =16.068,P=0.000).Auti-P53 level was also greater in HCC families featuring sibling affection than parental affection (x2 =12.679,P=0.002).Univariate analysis indicated that high expression of anti-P53 is a risk factor for development of HCC (OR=2.087,95％CI 1.270-3.431).Conclusion High level of anti-P53 expression may be a factor for the clustering of HCC families in Guangxi,China.