CAJ | 학술논문

以来自云南地方稻(Oryza sativa L.)种资源核心种质中的113份材料为研究对象,运用36对微卫星引物,研究了籼(indica)粳(japonica)两个亚种间和云南5个稻作生态区间的遗传多样性分布趋势,并筛选了籼粳亚种、水陆生态型和不同生态区的特异指纹标记.结果表明,粳稻的遗传多样性大于籼稻,遗传分化水平较低;而5个生态区中滇西南水陆稻区遗传多样性最大,遗传分化水平较低;滇东北高原粳稻区的遗传多样性最小.这种遗传多样性的分布趋势与前人在形态和同工酶水平上对云南稻种资源多样性的考察,以及云南地方稻种资源核心种质在形态和同工酶水平上的遗传多样性分布趋势基本一致.另外,在所出现的416个指纹标记中,分别发现籼粳特异指纹标记6个,水陆特异指纹标记15个,不同生态区特异指纹标记3个.初步认为,云南地方稻种资源核心种质代表了云南省地方稻种资源的遗传多样性;从DNA水平上看,云南地方稻种资源的遗传多样性中心在云南省的西南部,粳稻的分化水平低于籼稻.微卫星标记是种质资源遗传多样性检测、分类和生态型确认以及核心种质研究中有用的工具.
이래자운남지방도(Oryza sativa L.)충자원핵심충질중적113빈재료위연구대상,운용36대미위성인물,연구료선(indica)갱(japonica)량개아충간화운남5개도작생태구간적유전다양성분포추세,병사선료선갱아충、수륙생태형화불동생태구적특이지문표기.결과표명,갱도적유전다양성대우선도,유전분화수평교저;이5개생태구중전서남수륙도구유전다양성최대,유전분화수평교저;전동북고원갱도구적유전다양성최소.저충유전다양성적분포추세여전인재형태화동공매수평상대운남도충자원다양성적고찰,이급운남지방도충자원핵심충질재형태화동공매수평상적유전다양성분포추세기본일치.령외,재소출현적416개지문표기중,분별발현선갱특이지문표기6개,수륙특이지문표기15개,불동생태구특이지문표기3개.초보인위,운남지방도충자원핵심충질대표료운남성지방도충자원적유전다양성;종DNA수평상간,운남지방도충자원적유전다양성중심재운남성적서남부,갱도적분화수평저우선도.미위성표기시충질자원유전다양성검측、분류화생태형학인이급핵심충질연구중유용적공구.
The distribution of genetic diversity between Oryza sativa L. ssp. indica and O. sativa L. ssp. japonica covered different ecological zones in Yunnan was Studied, and the specific markers of indica/japonica subspecies, paddy/upland rice and different ecological zones were screened, using 36 microsatellite primers and 113 accessions in Yunnan landrace rice core collection. The results showed that the genetic diversity of japonica was higher than that of indica, and the ecological zone with the highest and smallest genetic diversity lay in Southeast and Northeast of Yunnan respectively. This distribution was consistent with the results of the studies on whole Yunnan rice resources and core collection at morphological and isozyme levels. In addition, the results showed that, among 416 markers, there were 6indica/japonica-specific markers, 15 specific markers in paddy/upland and 3 specific markers in different ecological zones. So the conclusion was primarily that the landrace rice core collection in Yunnan genetically represented the whole landrace rice resources in Yunnan, the center of genetic diversity at DNA level lay in Southeast of Yunnan, and the DNA differentiation between indica and japonica was small. And microsatellite marker was a useful tool to study the genetic diversity, classification and ecotype of germplasm resources and their core collection.