CAJ | 학술논문

目的了解重庆医科大学附一院近年临床分离的金葡菌中,耐消毒剂基因qacA的流行情况,从而指导临床抗菌药物、消毒剂的合理使用.方法选取有代表性的1价和2价化合物,检测126株金葡菌的MIC,阳性株用PCR法检测qacA基因型,采用CLSI推荐的30 μg头孢西丁纸片法进行MRSA鉴定.结果临床分离的126株金葡菌中,12株qacA基因型呈阳性(阳性率为9.5%),52株为MRSA(其中qacA基因阳性率为17.3%).结论临床分离的金葡菌中,qacA基因携带率较高.
목적 료해중경의과대학부일원근년림상분리적금포균중,내소독제기인qacA적류행정황,종이지도림상항균약물、소독제적합리사용.방법 선취유대표성적1개화2개화합물,검측126주금포균적MIC,양성주용PCR법검측qacA기인형,채용CLSI추천적30 μg두포서정지편법진행MRSA감정.결과 림상분리적126주금포균중,12주qacA기인형정양성(양성솔위9.5%),52주위MRSA(기중qacA기인양성솔위17.3%).결론 림상분리적금포균중,qacA기인휴대솔교고.
Objective To investigate the disinfectant-resistant gene qacA in Staphylococcus aureus isolates from the First Affiliated Hhospital of Chongqing Medical University. Methods A total of 126 S. aureus strains were isolated. The minimum inhibitory concentrations (MICs) of 4 representative monovalent and bivalent disinfectants were determined. Polymerase chain reaction (PCR) was employed to analyze the prevalence of qacA gene in these strains. Disk diffusion method was used to identify methicillin-resistant S. aureus (MRSA).Results Of the 126 S. aureus strains, 12 were positive for qacA gene. The prevalence of qacA in these strains was 9.5%. The prevalence of qacA gene in MRSA was 17.3%. Conclusions qacA gene is prevalent in the clinical isolates of S. aureus.