临床耳鼻咽喉头颈外科杂志
臨床耳鼻嚥喉頭頸外科雜誌
림상이비인후두경외과잡지
JOURNAL OF CLINICAL OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2009年
14期
646-648
,共3页
叶辉信%张锡流%欧海燕%黄卓燕
葉輝信%張錫流%歐海燕%黃卓燕
협휘신%장석류%구해연%황탁연
鼻咽肿瘤%E-钙黏蛋白%连环蛋白120%临床病理特征
鼻嚥腫瘤%E-鈣黏蛋白%連環蛋白120%臨床病理特徵
비인종류%E-개점단백%련배단백120%림상병리특정
nasopharyngeal neoplasms%E-cadherin%P120ctn%clinico-pathological features
目的:研究鼻咽癌组织中上皮钙黏蛋白(E-caol)和连环蛋白120(P120ctn)的表达情况,探讨它们的相关性及与临床病理特征的关系.方法:应用免疫组织化学二步法,检测经4%甲醛固定,石蜡包埋的56例鼻咽癌标本和15例正常鼻咽黏膜上皮标本中E-cad、P120ctn的表达情况.结果:56例鼻咽癌组织中E-cad、P120ctn 异常表达率分别为64.29%、67.86%,主要表现为细胞膜表达减弱,细胞膜表达减弱伴细胞胞质表达;15例对照组正常鼻咽黏膜上皮E-cad、P120ctn异常表达率均为6.67%,均差异有统计学意义(P<0.01).鼻咽癌组织中E-cad、P120ctn在低分化癌组的异常表达率(71.43%、85.71%)明显高于高中分化癌组(42.86%,36.29%);在有颈部淋巴结转移组的异常表达率(80.00%、85.00%)明显高于无颈部淋巴结转移组(52.78%、58.33%);在Ⅲ～Ⅳ期鼻咽癌组的异常表达率(76.92%、84.62%)明显高于Ⅰ～Ⅱ期鼻咽癌组(46.66%、53.33%);均差异有统计学意义(P<0.05).E-cad与P120ctn在56例鼻咽癌组织中共同表达和共同异常表达分别为12例和30例,有显著一致性,呈正相关(r=0.5217,P<0.01).结论:E-cad与P120ctn的异常表达与鼻咽癌的分化程度、临床分期、颈部淋巴结转移密切相关.它们共同参与鼻咽癌发生、发展、浸润及转移过程.
目的:研究鼻嚥癌組織中上皮鈣黏蛋白(E-caol)和連環蛋白120(P120ctn)的錶達情況,探討它們的相關性及與臨床病理特徵的關繫.方法:應用免疫組織化學二步法,檢測經4%甲醛固定,石蠟包埋的56例鼻嚥癌標本和15例正常鼻嚥黏膜上皮標本中E-cad、P120ctn的錶達情況.結果:56例鼻嚥癌組織中E-cad、P120ctn 異常錶達率分彆為64.29%、67.86%,主要錶現為細胞膜錶達減弱,細胞膜錶達減弱伴細胞胞質錶達;15例對照組正常鼻嚥黏膜上皮E-cad、P120ctn異常錶達率均為6.67%,均差異有統計學意義(P<0.01).鼻嚥癌組織中E-cad、P120ctn在低分化癌組的異常錶達率(71.43%、85.71%)明顯高于高中分化癌組(42.86%,36.29%);在有頸部淋巴結轉移組的異常錶達率(80.00%、85.00%)明顯高于無頸部淋巴結轉移組(52.78%、58.33%);在Ⅲ～Ⅳ期鼻嚥癌組的異常錶達率(76.92%、84.62%)明顯高于Ⅰ～Ⅱ期鼻嚥癌組(46.66%、53.33%);均差異有統計學意義(P<0.05).E-cad與P120ctn在56例鼻嚥癌組織中共同錶達和共同異常錶達分彆為12例和30例,有顯著一緻性,呈正相關(r=0.5217,P<0.01).結論:E-cad與P120ctn的異常錶達與鼻嚥癌的分化程度、臨床分期、頸部淋巴結轉移密切相關.它們共同參與鼻嚥癌髮生、髮展、浸潤及轉移過程.
목적:연구비인암조직중상피개점단백(E-caol)화련배단백120(P120ctn)적표체정황,탐토타문적상관성급여림상병리특정적관계.방법:응용면역조직화학이보법,검측경4%갑철고정,석사포매적56례비인암표본화15례정상비인점막상피표본중E-cad、P120ctn적표체정황.결과:56례비인암조직중E-cad、P120ctn 이상표체솔분별위64.29%、67.86%,주요표현위세포막표체감약,세포막표체감약반세포포질표체;15례대조조정상비인점막상피E-cad、P120ctn이상표체솔균위6.67%,균차이유통계학의의(P<0.01).비인암조직중E-cad、P120ctn재저분화암조적이상표체솔(71.43%、85.71%)명현고우고중분화암조(42.86%,36.29%);재유경부림파결전이조적이상표체솔(80.00%、85.00%)명현고우무경부림파결전이조(52.78%、58.33%);재Ⅲ～Ⅳ기비인암조적이상표체솔(76.92%、84.62%)명현고우Ⅰ～Ⅱ기비인암조(46.66%、53.33%);균차이유통계학의의(P<0.05).E-cad여P120ctn재56례비인암조직중공동표체화공동이상표체분별위12례화30례,유현저일치성,정정상관(r=0.5217,P<0.01).결론:E-cad여P120ctn적이상표체여비인암적분화정도、림상분기、경부림파결전이밀절상관.타문공동삼여비인암발생、발전、침윤급전이과정.
Objective:To study the expression of E-cadherin and P120ctn in nasopharyngeal carcinoma tissues, and to investigate their relationship and the relation with clinico-pathological features. Methods Two-step immuno-histochemical staining was applied to detect the expression of E-cadherin and P120ctn in formalin fixation and paraffin-embedded specimens from 56 cases with nasopharyngeal carcinoma and 15 cases with normal nasopharyngeal epithelia. Result:The abnormal expression rates of E-cadherin and P120ctn in the 56 cases of NPC tissues were 64.29% and 67.86% respectively, mainly with reduction of expression membrane and with the expression of cytoplasm; 6.67% of the 15 comparative normal cases of nasopharyngitis had abnormal expression of E-cadherin and P120ctn The differences were statistically significant. The abnormal expression rates of E-cadherin and P120ctn in NPC tissues were 71.43% and 85.71% respectively in low differentiated cancer group, which was obviously higher than the rates-42.86% and 36.29%-in high and middle differentiated cancer group. The 80.00% and 85.00% abnormal expression rate in the group with cervical lymph node metastases was higher than that in the group without cervical lymph node metastases(52.78%, 58.33%). The abnormal expression rate of E-cadherin and P120ctn(76. 92%,84.62%) in the third and forth phases was higher than that in the first and second phases (46.66%, 53. 33%). The differences were statistically significant (P<0.05). There were all together 12 co-expression cases of P120ctn and E-cadherin and 28 abnormal co-expression cases in the 56 cases of NPC tissues, which was of obvious consistency and correlation, with the relevant indexes: rs=0.5217 and P<0.01. Conclusion: The abnormal expression of E-cadherin and P120ctn is closely related to the degree of differentiation, clinical stage and cervical lymph node metastasis, and they join in the process of NPC initiation, progression, invasion and metastasis.
