CAJ | 학술논문

[目的]研究欧李脱毒快繁技术.[方法]以欧李嫩梢芽为外植体进行了微茎尖组培脱毒研究,探讨了在培养基中添加不同的激素成分及不同浓度对不定芽诱导、继代增殖和生根培养的影响.[结果]欧李茎尖脱毒快繁不定芽诱导的最佳培养基是:MS+6-BA 0.4 mg/L+IAA 0.5 mg/L;增殖培养的最适培养基为MS+6-BA 0.3 mg/L+IAA 0.4 mg/L; 最佳生根培养基是:2/3MS+IAA 0.5 mg/L.应用指示植物鉴定法进行病毒检测显示,0.3~0.4 mm茎尖培养对苹果褪绿斑病毒(ACLSV)和李矮缩病毒(PDV)的脱毒率分别为71.6%和73.0%.[结论]试验表明,利用欧李嫩梢为外植体进行茎尖组培脱毒快繁是一种可靠实用的方法.
[목적]연구구리탈독쾌번기술.[방법]이구리눈소아위외식체진행료미경첨조배탈독연구,탐토료재배양기중첨가불동적격소성분급불동농도대불정아유도、계대증식화생근배양적영향.[결과]구리경첨탈독쾌번불정아유도적최가배양기시:MS+6-BA 0.4 mg/L+IAA 0.5 mg/L;증식배양적최괄배양기위MS+6-BA 0.3 mg/L+IAA 0.4 mg/L; 최가생근배양기시:2/3MS+IAA 0.5 mg/L.응용지시식물감정법진행병독검측현시,0.3~0.4 mm경첨배양대평과퇴록반병독(ACLSV)화리왜축병독(PDV)적탈독솔분별위71.6%화73.0%.[결론]시험표명,이용구리눈소위외식체진행경첨조배탈독쾌번시일충가고실용적방법.
[Objective] The aim was to study the virvs-free and rapid propagation technique of Prunus humilis (Bunge.) [Method]Buds or tender stem of Prunus humilis as explant, the micropropagation was studied,the influence of the factors such as different hormone composition, concentration in culture medium upon adventitious bud induction, shoot proliferation and rooting were discussed. [Result] The result showed that the optimum medium for adventitious bud regeneration of virus-free and rapid propagation of Prunus humilis was MS+6-BA 0.4 mg/L +IAA 0.5 mg/L ;the best culture medium for shoot proliferation was MS+6-BA 0.3 mg/L+IAA 0.4 mg/L;the proper culture medium of rooting was 2/3 MS+IAA 0.5 mg/L .The virus-free rate of ACLSV and PDV were 71.6% and 73.0% respectively on 0.3-0.4 mm shoot tip based on the plant indicator identification, [Conclusion] The test showed that the virvs-free and rapid propagation technique of Prunus humilis (Bunge.) were tissue cultured by the method of buds or tender stem of Prunus humilis as explant which is a reliable and adapt method.