南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2010年
1期
88-91,95
,共5页
磷酸钙骨水泥%顺铂%氨磷汀%骨肉瘤细胞%药物缓释%骨缺损
燐痠鈣骨水泥%順鉑%氨燐汀%骨肉瘤細胞%藥物緩釋%骨缺損
린산개골수니%순박%안린정%골육류세포%약물완석%골결손
calcium phosphate cement%cisplatin%amifostine%osteosarcoma cell%drug delivery%bone defect
目的 对磷酸钙骨水泥/氨磷汀/顺铂(CPC/amifostine/cisplatin)复合体用作肿瘤性骨缺损的填充材料进行可行性研究.方法 按照质量比为1000:2:5的比例将磷酸钙骨水泥、顺铂、氨磷汀复合制备成人工骨复合体,对其进行固化时间、力学强度、孔隙率测定和扫描电镜、体外药物缓释、对骨肉瘤细胞的杀伤作用实验观察以及体内修复兔股骨骨缺损的实验研究.结果 CPC/amifostine/cisplatin复合体在固化时间、力学强度、孔隙率方面同单纯磷酸钙复合体相比没有差别,具有可持续缓释药物的作用,且氨磷汀的加入不影响顺铂对骨肉瘤细胞的毒性作用,在兔体内可观察到材料本身的降解和新骨长人材料内部的病理学证据.结论 CPC/amifostine/cisplatin复合体用作骨缺损的填充材料对于局部残存肿瘤细胞的消灭和缺损本身的修复是可行的.
目的 對燐痠鈣骨水泥/氨燐汀/順鉑(CPC/amifostine/cisplatin)複閤體用作腫瘤性骨缺損的填充材料進行可行性研究.方法 按照質量比為1000:2:5的比例將燐痠鈣骨水泥、順鉑、氨燐汀複閤製備成人工骨複閤體,對其進行固化時間、力學彊度、孔隙率測定和掃描電鏡、體外藥物緩釋、對骨肉瘤細胞的殺傷作用實驗觀察以及體內脩複兔股骨骨缺損的實驗研究.結果 CPC/amifostine/cisplatin複閤體在固化時間、力學彊度、孔隙率方麵同單純燐痠鈣複閤體相比沒有差彆,具有可持續緩釋藥物的作用,且氨燐汀的加入不影響順鉑對骨肉瘤細胞的毒性作用,在兔體內可觀察到材料本身的降解和新骨長人材料內部的病理學證據.結論 CPC/amifostine/cisplatin複閤體用作骨缺損的填充材料對于跼部殘存腫瘤細胞的消滅和缺損本身的脩複是可行的.
목적 대린산개골수니/안린정/순박(CPC/amifostine/cisplatin)복합체용작종류성골결손적전충재료진행가행성연구.방법 안조질량비위1000:2:5적비례장린산개골수니、순박、안린정복합제비성인공골복합체,대기진행고화시간、역학강도、공극솔측정화소묘전경、체외약물완석、대골육류세포적살상작용실험관찰이급체내수복토고골골결손적실험연구.결과 CPC/amifostine/cisplatin복합체재고화시간、역학강도、공극솔방면동단순린산개복합체상비몰유차별,구유가지속완석약물적작용,차안린정적가입불영향순박대골육류세포적독성작용,재토체내가관찰도재료본신적강해화신골장인재료내부적병이학증거.결론 CPC/amifostine/cisplatin복합체용작골결손적전충재료대우국부잔존종류세포적소멸화결손본신적수복시가행적.
Objective To invistigate the feasibility of using calcium phosphate cement/amifostine complex as an new filling material for reparing bone defect eansed by tumor resection. Methods Calcium phosphate cement (CPC)/cisplatin/amifostine complex was prepared at the mass ratio of 1000:2:5. The setting time, mechanical strength, and porosity of the complex were determined, and scanning electron microscopy and assessment of sustained drug release and inhibitory effect against osteosareoma cells were carried out. The degradiation of the material and new bone ingrowth were also observed in a rabbit model of femoral bone defect. Results The setting time, strength, and porosity, appearances under scanning electron microscope, and sustained drug release properties of CPC/cisplatin/amifostine complex were identical to those of CPC, and the integration of amifostine in the complex did not affect the cytotoxicity of eisplatin against the osteosarcoma cells. Pathological evidences of the degradiation of the material and new bone ingrowth into the material were observed with the passage of time following its implantation into the bone defect in rabbits. Conclusion The CPC/eisplatin/amifostine complex can be used as a filling material for repairing bone defect caused by tumor resection and eliminating the residual tumor cells in rabbits.
