CAJ | 학술논문

通过比较鸡IgG与鹅IgG的相似性,探讨羊抗鸡酶标二抗用于检测鹅血清抗体的可行性.用辛酸-硫酸铵盐析法提纯鸡、鸭、鹅、小鼠、免、猪、羊的IgG,比较上述动物IgG粗提物的SDS-聚丙烯酰胺凝胶电泳图谱,并用蛋白免疫转印法定性检测上述动物血清IgG与羊抗鸡酶标二抗的结合情况;然后用直接ELISA方法分析不同动物血清分别与抗鸡酶标抗体和抗鹅酶标抗体结合程度的差异;最后用间接EUSA方法比较两种酶标抗体对同一鹅群大肠杆菌抗体的检测结果.结果表明,鸡、鸭、鹅IgG具有较大的相似性,都能够与抗鸡酶标二抗结合;羊抗鸡酶标二抗可以替代抗鹅酶标二抗来检测鹅的血清抗体.
통과비교계IgG여아IgG적상사성,탐토양항계매표이항용우검측아혈청항체적가행성.용신산-류산안염석법제순계、압、아、소서、면、저、양적IgG,비교상술동물IgG조제물적SDS-취병희선알응효전영도보,병용단백면역전인법정성검측상술동물혈청IgG여양항계매표이항적결합정황;연후용직접ELISA방법분석불동동물혈청분별여항계매표항체화항아매표항체결합정도적차이;최후용간접EUSA방법비교량충매표항체대동일아군대장간균항체적검측결과.결과표명,계、압、아IgG구유교대적상사성,도능구여항계매표이항결합;양항계매표이항가이체대항아매표이항래검측아적혈청항체.
The mechanism of detecting antibody levels in goose serum samples with goat-anti-chicken IgG was clarified by comparing the similarity of chicken IgG with goose IgG.Chicken IgG,duck IgG,goose IgG,mouse IgG,rabbit IgG,pig IgG and goat IgG were salted out by octanoic acid-saturated ammonium sulfate.Their SDS-PAGE pattern was analyzed and the binding was qualitative checked up through western blotting.The binding difference of chicken IgG,duck IgG,goose IgG,mouse IgG,rabbit IgG,pig IgG and goat IgG to anti-chicken-IgG and anti-goose-IgG,were assayed by direct ELISA.Finally the binding difference of two different secondary antibodies to blood-serum of the same group was assayed by indirect ELISA.The results showed that there was much resemblance between chicken IgG,duck IgG and goose IgG,they could also bind to anti-chicken-IgG;Goat-anti-chicken secondary antibody could replace anti-goose IgG to diagnose detecting antibody levels in goose serum.