CAJ | 학술논문

背景:冷冻干燥血管是进行血管移植的良好材料,但目前对冷冻干燥工艺的研究不多,同时缺乏对冻干后血管物理学性能变化的分析报道.目的:利用冷冻干燥的方法对血管的保存作了初步尝试,使用微型CT对冻干过程进行跟踪,以揭示冻干过程中血管形态发生的变化.设计及地点:单一样本观察,实验在上海理工大学制冷及低温研究所完成.材料:上海屠宰场生猪新鲜主动脉.冷冻干燥机为美国SP Industries公司生产;TRAPEZIUM LITE质构仪由岛津(香港)有限公司生产.方法:使用质构仪对冻干后复水的猪主动脉与新鲜猪主动脉进行力学性能对比分析.主要观察指标:①冷冻干燥过程温度曲线.②微CT断层扫描猪主动脉冻干过程.③猪主动脉冻干前后拉伸力学性能及穿刺力学性能比较.结果:试验结果发现猪主动脉血管在冻干过程中会发生分层现象,血管冻干后复水,其力学性能同新鲜血管比最大轴向拉伸应力减少约40%.,周向最大拉伸应力增大约45%,最大穿刺应力约是新鲜血管的75%.结论:冻干复水后的血管基本保持了新鲜血管的力学性能,可望成为一种有效的血管保存手段.
배경:냉동간조혈관시진행혈관이식적량호재료,단목전대냉동간조공예적연구불다,동시결핍대동간후혈관물이학성능변화적분석보도.목적:이용냉동간조적방법대혈관적보존작료초보상시,사용미형CT대동간과정진행근종,이게시동간과정중혈관형태발생적변화.설계급지점:단일양본관찰,실험재상해리공대학제랭급저온연구소완성.재료:상해도재장생저신선주동맥.냉동간조궤위미국SP Industries공사생산;TRAPEZIUM LITE질구의유도진(향항)유한공사생산.방법:사용질구의대동간후복수적저주동맥여신선저주동맥진행역학성능대비분석.주요관찰지표:①냉동간조과정온도곡선.②미CT단층소묘저주동맥동간과정.③저주동맥동간전후랍신역학성능급천자역학성능비교.결과:시험결과발현저주동맥혈관재동간과정중회발생분층현상,혈관동간후복수,기역학성능동신선혈관비최대축향랍신응력감소약40%.,주향최대랍신응력증대약45%,최대천자응력약시신선혈관적75%.결론:동간복수후적혈관기본보지료신선혈관적역학성능,가망성위일충유효적혈관보존수단.
BACKGROUND: The freeze-drying blood vessel is an excellent material for vasotransplantation. However, the reports concerning freeze-drying are few, and the mechanical property changes of freeze-dried blood vessel remains poorly understood. OBJECTIVE: Using micro-CT to explore the morphological changes of blood vessel during procedure of freeze-drying. DESIGN, TIME AND SETTING: A single sample observation was performed at the Institute of Refrigeration and Cryogenics, University of Shanghai for Science and Technology. MATERIALS: Fresh pig aorta was obtained from Shanghai Slaughter House. The lyophilizer was produced by SP Industries (USA). TRAPEZIUM LITE texture analyzer was purchased from Shimadzu (Hong Kong) Co., Ltd.METHODS: The differences of mechanical property between fresh aorta and freeze-dried aorta were compared.MAIN OUTCOME MEASURES: ① Temperature curve of freeze-drying procedure. ②The reeze-drying procedure was scanned by micro-CT. ③ Changes of mechanical property were detected by TRAPEZIUM LITE texture analyzer and puncture experiments.RESULTS: During freeze-drying procedure, there were presented layer phenomenon in pig aorta. Compared to the fresh aorta, the freeze-dried aorta after recover water would reduce 40% axial tensile force, increase 45% circumferential tensile force, with 75% puncture stress.CONCLUSION: The freeze-dried blood vessel maintains mechanical property as fresh vessel, thus, freeze-drying can be used for vascular preservation.