CAJ | 학술논문

目的研究临床上常用8种试剂对血液样品中碳氧血红蛋门(HbCO)稳定性的影响.方法将血液样品分为高、低两个HbCO浓度组,选用临床常用的甲醛、氟化钠、乙二胺四乙酸二钠、亚硝酸钠、草酸钾、肝素钠、柠檬酸钾及氟化钠与草酸钾混合物(1:3)8种试剂,按常用浓度分别添加到血样品中,并于添加后0h、2h、8h、24h、3d、7d用紫外-可见光分光光度法检测其中HbCO饱和度,用统计学方法进行结果分析.结果本实验选用的8种试剂只有甲醛和亚硝酸钠对HbCO的稳定性影响较为显著,而其余6种对HbCO稳定性的影响无统计学意义.结论在检验疑似CO中毒并经甲醛或亚硝酸钠防腐的检材时应慎重,以免导致错误的鉴定结论.
목적 연구림상상상용8충시제대혈액양품중탄양혈홍단문(HbCO)은정성적영향.방법 장혈액양품분위고、저량개HbCO농도조,선용림상상용적갑철、불화납、을이알사을산이납、아초산납、초산갑、간소납、저몽산갑급불화납여초산갑혼합물(1:3)8충시제,안상용농도분별첨가도혈양품중,병우첨가후0h、2h、8h、24h、3d、7d용자외-가견광분광광도법검측기중HbCO포화도,용통계학방법진행결과분석.결과 본실험선용적8충시제지유갑철화아초산납대HbCO적은정성영향교위현저,이기여6충대HbCO은정성적영향무통계학의의.결론 재검험의사CO중독병경갑철혹아초산납방부적검재시응신중,이면도치착오적감정결론.
Objective To investigate the effect of conventional preseratives on the stability of carboxyhemoglobin(HbCO)in the stored blood samples.Methods Blood samples with 30%～40% and 60%～70% of HbCO were established,respectiviely.The samples were stored with the commomly used preseratives including formaldehyde,sodium fluoride,EDTA-Na_2,sodium nitrite,potassium oxalate,heparin sodium,sodium citrate and the mixture of sodium fluoride and sodium citrate(1:3).Saturation of HbCO in the preserved blood samples were determined at 0h,2h,8h,24h,3d,and 7d after treatment with the addictives,respectively.The data were evaluated statistically.Results The stability of HbCO was significantly affected in the formaldehyde and sodium nitrite-treated samples,but no significant effects of the other preseratives on the blood samples were detected.Conclusion The stability of HbCO in the blood samples conserved varies with the type of the preseratives used.The samples taken from cases with suspected death from carbon monoxide poisoning should be kept with proper preseratives.Blood samples preserved with formaldehyde and sodium nitrite are not suitable for HbCO determination.