CAJ | 학술논문

背景:肌源性干细胞作为种子细胞用于制备组织工程化人工神经已经被越来越多的学者所接受.他克莫司不仅具有抗免疫排斥的效果,还具有强大的促进神经再生和修复的作用.那么能否将两项因素与去细胞异体神经支架形成一个桥接体,既能抑制异体移植的免疫反应,又能有效促进损伤神经的再生与修复?目的:采用理化联合处理方法制备去细胞异体神经支架,探讨肌源性干细胞与免疫抑制剂他克莫司联合应用对去细胞异体神经支架移植后神经再生及功能恢复的影响.方法:SD大鼠坐骨神经经脱细胞处理后形成异体神经桥接体.用100 pL微量注射器将含他克莫司与肌源性干细胞的凝胶注入异体神经支架,用以修复大鼠的坐骨神经缺损.32只成年SD大鼠,随机分为4组,每组8只.切断其左侧坐骨神经造成10 mm的缺损.他克莫司+肌源性干细胞组、肌源性干细胞组、他克莫司组以注射植入后的异体神经进行桥接;对照组仅注入透明质酸凝胶.术后8,12周进行坐骨神经指数和神经电生理测定.术后12周进行大体观察,神经组织学和超微结构观察.结果和结论:在同一时点他克莫司+肌源性干细胞组坐骨神经功能指数、坐骨神经运动传导速度恢复率、移植体及远段有髓纤维计数均优于其他3组(P<0.05).各组神经移植体粗细基本正常,表面大量血管分布,与周围组织轻度粘连;他克莫司+肌源性干细胞组较其他3组再生神经纤维更加密集、排列规则整齐;移植体许旺细胞大量增殖,移植体中央及远段内的有髓神经纤维密度、直径高于肌源性干细胞组、他克莫司组,微束之间结缔组织少,接近于正常.说明肌源性干细胞和他克莫司联合应用促进去细胞异种神经移植的神经再生与功能恢复的效果优于单独应用.肌源性干细胞和他克莫司在周围神经损伤修复中是一对具有协同作用的因子. 揹景:肌源性榦細胞作為種子細胞用于製備組織工程化人工神經已經被越來越多的學者所接受.他剋莫司不僅具有抗免疫排斥的效果,還具有彊大的促進神經再生和脩複的作用.那麽能否將兩項因素與去細胞異體神經支架形成一箇橋接體,既能抑製異體移植的免疫反應,又能有效促進損傷神經的再生與脩複?目的:採用理化聯閤處理方法製備去細胞異體神經支架,探討肌源性榦細胞與免疫抑製劑他剋莫司聯閤應用對去細胞異體神經支架移植後神經再生及功能恢複的影響.方法:SD大鼠坐骨神經經脫細胞處理後形成異體神經橋接體.用100 pL微量註射器將含他剋莫司與肌源性榦細胞的凝膠註入異體神經支架,用以脩複大鼠的坐骨神經缺損.32隻成年SD大鼠,隨機分為4組,每組8隻.切斷其左側坐骨神經造成10 mm的缺損.他剋莫司+肌源性榦細胞組、肌源性榦細胞組、他剋莫司組以註射植入後的異體神經進行橋接;對照組僅註入透明質痠凝膠.術後8,12週進行坐骨神經指數和神經電生理測定.術後12週進行大體觀察,神經組織學和超微結構觀察.結果和結論:在同一時點他剋莫司+肌源性榦細胞組坐骨神經功能指數、坐骨神經運動傳導速度恢複率、移植體及遠段有髓纖維計數均優于其他3組(P<0.05).各組神經移植體粗細基本正常,錶麵大量血管分佈,與週圍組織輕度粘連;他剋莫司+肌源性榦細胞組較其他3組再生神經纖維更加密集、排列規則整齊;移植體許旺細胞大量增殖,移植體中央及遠段內的有髓神經纖維密度、直徑高于肌源性榦細胞組、他剋莫司組,微束之間結締組織少,接近于正常.說明肌源性榦細胞和他剋莫司聯閤應用促進去細胞異種神經移植的神經再生與功能恢複的效果優于單獨應用.肌源性榦細胞和他剋莫司在週圍神經損傷脩複中是一對具有協同作用的因子.
배경:기원성간세포작위충자세포용우제비조직공정화인공신경이경피월래월다적학자소접수.타극막사불부구유항면역배척적효과,환구유강대적촉진신경재생화수복적작용.나요능부장량항인소여거세포이체신경지가형성일개교접체,기능억제이체이식적면역반응,우능유효촉진손상신경적재생여수복?목적:채용이화연합처리방법제비거세포이체신경지가,탐토기원성간세포여면역억제제타극막사연합응용대거세포이체신경지가이식후신경재생급공능회복적영향.방법:SD대서좌골신경경탈세포처리후형성이체신경교접체.용100 pL미량주사기장함타극막사여기원성간세포적응효주입이체신경지가,용이수복대서적좌골신경결손.32지성년SD대서,수궤분위4조,매조8지.절단기좌측좌골신경조성10 mm적결손.타극막사+기원성간세포조、기원성간세포조、타극막사조이주사식입후적이체신경진행교접;대조조부주입투명질산응효.술후8,12주진행좌골신경지수화신경전생리측정.술후12주진행대체관찰,신경조직학화초미결구관찰.결과화결론:재동일시점타극막사+기원성간세포조좌골신경공능지수、좌골신경운동전도속도회복솔、이식체급원단유수섬유계수균우우기타3조(P<0.05).각조신경이식체조세기본정상,표면대량혈관분포,여주위조직경도점련;타극막사+기원성간세포조교기타3조재생신경섬유경가밀집、배렬규칙정제;이식체허왕세포대량증식,이식체중앙급원단내적유수신경섬유밀도、직경고우기원성간세포조、타극막사조,미속지간결체조직소,접근우정상.설명기원성간세포화타극막사연합응용촉진거세포이충신경이식적신경재생여공능회복적효과우우단독응용.기원성간세포화타극막사재주위신경손상수복중시일대구유협동작용적인자.
BACKGROUND: Muscle-derived stem cells (MDSCs) have been accepted as seeding cells in tissue engineered artificial nerves. Tacrolimus exhibits anti-immunologic rejection and promotes nerve regeneration and recovery. Whether can combine these factors with acellular nerve to form a new bridge that can inhibit immunologic rejection and promote nerve regeneration is uncertain. OBJECTIVE: Using the freeze-thawing combined optimized acellular nerve hypotonic-chemical detergent to prepare acellular nerveallograft scaffold. To explore the effect of co-application of MDSCs and FK-506 on nerve regeneration and function recovery following acellular nerve ailograft implantation.METHODS: The sciatic nerve derived from SD rats was prepared nerve bridge after acallular disposal. Gel containing FK-506 and MDSCs was injected into acellular nerveallograft scaffold with 100 μL microsyringe to repair defects. A total of 32 SD rats were randomly divided into 4 groups, with 8 animals in each group. Agap of 10 mm of left sciatic nerve was removed. And then the defects were repaired by extracted nerve graft containing FK-506+MDSCs, MDSCs and FK-506, respectively. In the control group, only hyaluronic acid gel was injected. Sciatic nerve function index (SFI) and electrophysiological exam were performed at weeks 8 and 12 after operation. Gross observation, neurohistological and ultrastructure were observed at week 12 after operation.RESULTS AND CONCLUSION: Compared with the same time point, the SFI, recovery rate of the motor nerve conduction velocities (MNCV), and myelinated nerve fibers in grafting part and in its distal part in the FK-506+MDSCs group were superior to other groups (P < 0.05). The nerve grafts were in normal size with considerabie blood vessels and slightly connected to peripheral Ussues. Compared to other groups, the regenerated nerve fiber in the FK-506+MDSCs group was more density with well-arranged order. A great quantity of Schwann cells proliferated in grafting. The density end diameter of myelinated fiber in the middle and distal part of the grafting were all greater than that of MDSCs and FK-506 groups, and there were few connective tissues between microfascicles, which was close to normal level. The co-application of MDSCs and FK-506 enhances peripheral nerve regeneration and functional recovery in acelluler nerve allograft graft. Therefore, MDSCs and FK-506 are synergistic factors in peripheral nerve injury repair.