广东化工
廣東化工
엄동화공
GUANGDONG CHEMICAL INDUSTRY
2012年
3期
156-157
,共2页
气相色谱仪%植物性食品%氯丹%残留量
氣相色譜儀%植物性食品%氯丹%殘留量
기상색보의%식물성식품%록단%잔류량
liquid chromatography coupled with eletrospray ionization quadrupole tandem mass spectrum etry%aquaculture water： chloramphenicol
建立用气相色谱仪法快速测定植物性食品中氯丹残留量。试样经丙酮提取，正己烷萃取，使用HP-5色谱柱分离，ECD测定。结果表明，顺式氯丹、反式氯丹与氧氯丹能够很好分离，在0．2-100pg，L线性关系良好（r≥O．999）。在2．0-10．0．g／kg的添加水平范围内的平均回收率为82．8％-94．6％，相对标准偏差为2．0％-6．5％。顺式氯丹、反式氯丹与氧氯丹该方法检出限均为0．5gg／kg。
建立用氣相色譜儀法快速測定植物性食品中氯丹殘留量。試樣經丙酮提取，正己烷萃取，使用HP-5色譜柱分離，ECD測定。結果錶明，順式氯丹、反式氯丹與氧氯丹能夠很好分離，在0．2-100pg，L線性關繫良好（r≥O．999）。在2．0-10．0．g／kg的添加水平範圍內的平均迴收率為82．8％-94．6％，相對標準偏差為2．0％-6．5％。順式氯丹、反式氯丹與氧氯丹該方法檢齣限均為0．5gg／kg。
건립용기상색보의법쾌속측정식물성식품중록단잔류량。시양경병동제취，정기완췌취，사용HP-5색보주분리，ECD측정。결과표명，순식록단、반식록단여양록단능구흔호분리，재0．2-100pg，L선성관계량호（r≥O．999）。재2．0-10．0．g／kg적첨가수평범위내적평균회수솔위82．8％-94．6％，상대표준편차위2．0％-6．5％。순식록단、반식록단여양록단해방법검출한균위0．5gg／kg。
A high liquid chromatography coupled with eletrospray ionization quadrupole tandem mass spectrum etry rapid determination method of chloramphenicol residues in aquaculture water. An ultra Performance liquid chromatography coupled with eletrospray ionization quadrupole tandem mass spectrum etry was used. The residue was quantified with multiple reactiong monitoring （MRM） mode. The method was validated and good results were obtained with respect to precision, repeatability and spiked recovery. The limit of detection was 0.005 p-g/L for chloramphenicol, and the linear range was from 0.2 to 100 μg/L. The average recoveries were between 82.8 % to 94.6 % in the spiked range of 0.05-0.5 μg/L, and the relative standard deviations（RSD） were between 2.0 % to 6.5 %. The method has good repeatability and high sensitivity （r=0.999）, and can be applied for the determination of chloramphenicol residue in aquaculture water.
