中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2012年
9期
676-680
,共5页
梁琳琳%刘玉青%李杭生%张翠莲
樑琳琳%劉玉青%李杭生%張翠蓮
량림림%류옥청%리항생%장취련
玻璃化冷冻%低温保存%胚胎移植%卵巢%生长分化因子9%小鼠
玻璃化冷凍%低溫保存%胚胎移植%卵巢%生長分化因子9%小鼠
파리화냉동%저온보존%배태이식%란소%생장분화인자9%소서
Vitrification%Cryopreservation%Embryo transfer%Ovary%Growth differentiation factor 9%Mice
目的 探讨玻璃化冷冻胚胎出生小鼠的卵巢发育情况及卵巢组织中生长分化因子9(GDF-9)的表达.方法 将玻璃化冷冻复苏胚胎(玻璃化冷冻组)和新鲜胚胎(新鲜胚胎组)分别移植入假孕小鼠子宫内,两组出生后的雌性子鼠分别于第3、7、14、21、28、60天处死,取卵巢组织,每组每个时间点各6只子鼠.对卵巢组织采用HE染色观察卵巢发育情况,采用荧光定量逆转录(RT)PCR技术和蛋白印迹法检测卵巢组织中生长分化因子9(GDF-9) mRNA和蛋白的表达水平.结果 HE染色结果显示,两组子鼠卵巢发育无明显异常.子鼠出生后第3、7、14、21、28、60天,玻璃化冷冻组子鼠卵巢组织中GDF-9 mRNA表达水平分别为:0.14±0.07、0.42±0.16、1.00±0.24、1.59±0.28、2.05±0.32、2.23±0.21,新鲜胚胎组分别为:0.13±0.06、0.45±0.18、1.00±0.21、1.56±0.26、2.01±0.37、2.26±0.23,两组分别比较,差异均无统计学意义(P＞0.05)；玻璃化冷冻组各时间点子鼠卵巢组织中GDF-9蛋白表达水平分别为:0.040±0.030、0.120±0.060、0.170±0.030、0.250±0.040、0.320±0.060、0.330±0.010,新鲜胚胎组分别为:0.030±0.020、0.110±0.040、0.150 ±0.010、0.210±0.020、0.360±0.070、0.350±0.030,两组分别比较,差异均无统计学意义(P＞0.05).结论玻璃化冷冻胚胎出生小鼠的卵巢形态及GDF-9表达无明显变化.
目的 探討玻璃化冷凍胚胎齣生小鼠的卵巢髮育情況及卵巢組織中生長分化因子9(GDF-9)的錶達.方法 將玻璃化冷凍複囌胚胎(玻璃化冷凍組)和新鮮胚胎(新鮮胚胎組)分彆移植入假孕小鼠子宮內,兩組齣生後的雌性子鼠分彆于第3、7、14、21、28、60天處死,取卵巢組織,每組每箇時間點各6隻子鼠.對卵巢組織採用HE染色觀察卵巢髮育情況,採用熒光定量逆轉錄(RT)PCR技術和蛋白印跡法檢測卵巢組織中生長分化因子9(GDF-9) mRNA和蛋白的錶達水平.結果 HE染色結果顯示,兩組子鼠卵巢髮育無明顯異常.子鼠齣生後第3、7、14、21、28、60天,玻璃化冷凍組子鼠卵巢組織中GDF-9 mRNA錶達水平分彆為:0.14±0.07、0.42±0.16、1.00±0.24、1.59±0.28、2.05±0.32、2.23±0.21,新鮮胚胎組分彆為:0.13±0.06、0.45±0.18、1.00±0.21、1.56±0.26、2.01±0.37、2.26±0.23,兩組分彆比較,差異均無統計學意義(P＞0.05)；玻璃化冷凍組各時間點子鼠卵巢組織中GDF-9蛋白錶達水平分彆為:0.040±0.030、0.120±0.060、0.170±0.030、0.250±0.040、0.320±0.060、0.330±0.010,新鮮胚胎組分彆為:0.030±0.020、0.110±0.040、0.150 ±0.010、0.210±0.020、0.360±0.070、0.350±0.030,兩組分彆比較,差異均無統計學意義(P＞0.05).結論玻璃化冷凍胚胎齣生小鼠的卵巢形態及GDF-9錶達無明顯變化.
목적 탐토파리화냉동배태출생소서적란소발육정황급란소조직중생장분화인자9(GDF-9)적표체.방법 장파리화냉동복소배태(파리화냉동조)화신선배태(신선배태조)분별이식입가잉소서자궁내,량조출생후적자성자서분별우제3、7、14、21、28、60천처사,취란소조직,매조매개시간점각6지자서.대란소조직채용HE염색관찰란소발육정황,채용형광정량역전록(RT)PCR기술화단백인적법검측란소조직중생장분화인자9(GDF-9) mRNA화단백적표체수평.결과 HE염색결과현시,량조자서란소발육무명현이상.자서출생후제3、7、14、21、28、60천,파리화냉동조자서란소조직중GDF-9 mRNA표체수평분별위:0.14±0.07、0.42±0.16、1.00±0.24、1.59±0.28、2.05±0.32、2.23±0.21,신선배태조분별위:0.13±0.06、0.45±0.18、1.00±0.21、1.56±0.26、2.01±0.37、2.26±0.23,량조분별비교,차이균무통계학의의(P＞0.05)；파리화냉동조각시간점자서란소조직중GDF-9단백표체수평분별위:0.040±0.030、0.120±0.060、0.170±0.030、0.250±0.040、0.320±0.060、0.330±0.010,신선배태조분별위:0.030±0.020、0.110±0.040、0.150 ±0.010、0.210±0.020、0.360±0.070、0.350±0.030,량조분별비교,차이균무통계학의의(P＞0.05).결론파리화냉동배태출생소서적란소형태급GDF-9표체무명현변화.
Objective To study ovarian development in vitrificatiou of embryos born mice and expression of growth differentiation factor 9 ( GDF-9 ) in its.Methods The vitrification recovery embryos (vitrified-embryo group) and fresh embryos (fresh-embryo group) were transplanted into pseudopregnant mice,respectively.The female offspring mice in two groups were sacrificcd on the 3rd,7th,14th,21st,28th and 60th day after birth,the ovarian tissues were taken,6 mice in each time point of each group.The ovarian development was observed by HE staining,the expression of GDF-9 mRNA and protein at each time point of two groups were detected by reverse transcription(RT)-PCR and western blot.Results HE staining showed that no abnormal ovarian development was observed in offsprings at two groups.On the 3rd,7th,14th,21st,28th and 60th day after birth,the expression of GDF-9 mRNA in vitrified-embryo group were 0.14 ± 0.07,0.42±0.16,1.00±0.24,1.59±0.28,2.05 ±0.32 and 2.23 ±0.21,respectively,which in fresh-embryo group were 0.13 ±0.06,0.45 ±0.18,1.00 ±0.21,1.56 ±0.26,2.01 0.37 and 2.26 ±0.23,respectively,there was no statistical difference between two groups ( P ＞ 0.05 ) ; the expression of GDF-9 protein in vitrified-embryo group were 0.040 ± 0.030,0.120 ± 0.060,0.170 ± 0.030,0.250 ± 0.040,0.320± 0.060 and 0.330 ± 0.010,respectively,which in fresh-embryo group were 0.030 ± 0.020,0.110 ± 0.040,0.150 ± 0.010,0.210 ± 0.020,0.360 ± 0.070 and 0.350 ± 0.030,respectively,there was no statistical difference between two groups ( P ＞ 0.05 ).Conclusion The ovarian morphology in vitrification of embryos born mice and expression of GDF-9 in ovary has no any obvious change.
