中华眼视光学与视觉科学杂志
中華眼視光學與視覺科學雜誌
중화안시광학여시각과학잡지
CHINESE JOURNAL OF OPTOMETRY OPHTHALMOLOGY AND VISUAL SCIENCE
2011年
6期
416-418
,共3页
人工泪液%视网膜电描记术%SD大鼠%振幅
人工淚液%視網膜電描記術%SD大鼠%振幅
인공루액%시망막전묘기술%SD대서%진폭
Artificial tear%Electroretinography%SD rat%Amplitude
目的 观察人工泪液对大鼠视网膜电图(ERG)的影响.方法 动物实验研究.SD大鼠充分暗适应后,记录单眼ERG.ERG记录采用自制银丝电极和不锈钢针状电极,其中银丝电极为记录电极,置于角巩膜缘,各组ERG记录眼人工泪液用量不同:第1组为未滴人工泪液组；第2组为适量组,滴少量人工泪液,使电极与角巩膜缘接触良好；第3组为过量组,滴入大量人工泪液,使之充满整个结膜囊.ERG a、b波幅值和峰时采用单因素方差分析比较组间差异,两两间差异采用LSDt法进行比较.结果 使用人工泪液的两组动物ERG基线平滑,干扰波不明显；然而人工泪液使用过量组a、b波振幅均明显降低,与其他两组相比差异均有统计学意义(t=4.112,P＜0.01；t=3.018,P＜0.05).结论 视觉电生理实验室经屏蔽和有效接地后,动物ERG记录中使用人工泪液可以进一步降低干扰,但人工泪液使用过量会影响ERG振幅大小.
目的 觀察人工淚液對大鼠視網膜電圖(ERG)的影響.方法 動物實驗研究.SD大鼠充分暗適應後,記錄單眼ERG.ERG記錄採用自製銀絲電極和不鏽鋼針狀電極,其中銀絲電極為記錄電極,置于角鞏膜緣,各組ERG記錄眼人工淚液用量不同:第1組為未滴人工淚液組；第2組為適量組,滴少量人工淚液,使電極與角鞏膜緣接觸良好；第3組為過量組,滴入大量人工淚液,使之充滿整箇結膜囊.ERG a、b波幅值和峰時採用單因素方差分析比較組間差異,兩兩間差異採用LSDt法進行比較.結果 使用人工淚液的兩組動物ERG基線平滑,榦擾波不明顯；然而人工淚液使用過量組a、b波振幅均明顯降低,與其他兩組相比差異均有統計學意義(t=4.112,P＜0.01；t=3.018,P＜0.05).結論 視覺電生理實驗室經屏蔽和有效接地後,動物ERG記錄中使用人工淚液可以進一步降低榦擾,但人工淚液使用過量會影響ERG振幅大小.
목적 관찰인공루액대대서시망막전도(ERG)적영향.방법 동물실험연구.SD대서충분암괄응후,기록단안ERG.ERG기록채용자제은사전겁화불수강침상전겁,기중은사전겁위기록전겁,치우각공막연,각조ERG기록안인공루액용량불동:제1조위미적인공루액조；제2조위괄량조,적소량인공루액,사전겁여각공막연접촉량호；제3조위과량조,적입대량인공루액,사지충만정개결막낭.ERG a、b파폭치화봉시채용단인소방차분석비교조간차이,량량간차이채용LSDt법진행비교.결과 사용인공루액적량조동물ERG기선평활,간우파불명현；연이인공루액사용과량조a、b파진폭균명현강저,여기타량조상비차이균유통계학의의(t=4.112,P＜0.01；t=3.018,P＜0.05).결론 시각전생리실험실경병폐화유효접지후,동물ERG기록중사용인공루액가이진일보강저간우,단인공루액사용과량회영향ERG진폭대소.
Objective To observe the effect of artificial tear solution on the electroretinogram (ERG) of SD rats.Methods Fifteen adult SD rats were divided into 3 groups in this experimental study.After 6 hours of dark adaptation,a silver loop electrode was placed in contact with the cornea as the recording electrode. Stainless steel needles were used as reference and ground electrode.Different amounts of artificial tear (1％ carboxymethylcellulose sodium) were instilled in the recording eye: group one: the cornea was kept dry without artificial tear; group two: the amount of artificial tear was moderate; group three: the amount was excessive. ERG was recorded and the a- and b-waves were monitored.Data were analyzed with a one-way ANOVA and differeces between each group were compared by the LSD t test.Results In groups two and three, the interference was subtle and the baseline was smooth compared to group one. There was a statistically significant decrease in the a- and b-wave amplitudes in group three (t=4.112, P＜0.01; t=3.018,P＜0.05).Conclusion Artificial tear solution can reduce interference in ERG recordings but excessive amounts may affect the amplitude of the waves.
