中华显微外科杂志
中華顯微外科雜誌
중화현미외과잡지
Chinese Journal of Microsurgery
2010年
6期
461-464,后插7
,共5页
人脐带%间充质干细胞%去细胞神经基膜管%组织工程%种子细胞
人臍帶%間充質榦細胞%去細胞神經基膜管%組織工程%種子細胞
인제대%간충질간세포%거세포신경기막관%조직공정%충자세포
Human umbilical cord%Mesenchymal stem cells%Acellular nerve basal lamina tube%Tissue engineering%Seed cells
目的 探讨诱导后的脐带间充质干细胞作为组织工程种子细胞修复大鼠坐骨神经缺损的可行性.方法 从正常足月新生儿脐带中分离培养间充质干细胞并诱导分化为神经样细胞,与去细胞神经基膜管共培养以构建组织工程神经;用30只健康成年雄性SD大鼠建立坐骨神经缺损(10 mm)的动物模型并随机分成3组:A组为脐带间充质干细胞复合去细胞神经基膜管组,B组为单纯去细胞神经基膜管组,C组为自体神经桥接组.术后10周通过神经电生理检测、组织学观察等评测效果.结果 在局部观察和肌肉测量、神经电生理检测、组织学观察等方面,脐带间充质干细胞复合去细胞神经基膜管组(A组)神经再生及肢体功能情况良好,效果接近于自体神经桥接组(C组),明显优于单纯去细胞神经基膜管组(B组).结论 脐带间充质干细胞复合去细胞神经基膜管构建的组织工程神经可有效促进大鼠坐骨神经缺损(10 mm)的修复.
目的 探討誘導後的臍帶間充質榦細胞作為組織工程種子細胞脩複大鼠坐骨神經缺損的可行性.方法 從正常足月新生兒臍帶中分離培養間充質榦細胞併誘導分化為神經樣細胞,與去細胞神經基膜管共培養以構建組織工程神經;用30隻健康成年雄性SD大鼠建立坐骨神經缺損(10 mm)的動物模型併隨機分成3組:A組為臍帶間充質榦細胞複閤去細胞神經基膜管組,B組為單純去細胞神經基膜管組,C組為自體神經橋接組.術後10週通過神經電生理檢測、組織學觀察等評測效果.結果 在跼部觀察和肌肉測量、神經電生理檢測、組織學觀察等方麵,臍帶間充質榦細胞複閤去細胞神經基膜管組(A組)神經再生及肢體功能情況良好,效果接近于自體神經橋接組(C組),明顯優于單純去細胞神經基膜管組(B組).結論 臍帶間充質榦細胞複閤去細胞神經基膜管構建的組織工程神經可有效促進大鼠坐骨神經缺損(10 mm)的脩複.
목적 탐토유도후적제대간충질간세포작위조직공정충자세포수복대서좌골신경결손적가행성.방법 종정상족월신생인제대중분리배양간충질간세포병유도분화위신경양세포,여거세포신경기막관공배양이구건조직공정신경;용30지건강성년웅성SD대서건립좌골신경결손(10 mm)적동물모형병수궤분성3조:A조위제대간충질간세포복합거세포신경기막관조,B조위단순거세포신경기막관조,C조위자체신경교접조.술후10주통과신경전생리검측、조직학관찰등평측효과.결과 재국부관찰화기육측량、신경전생리검측、조직학관찰등방면,제대간충질간세포복합거세포신경기막관조(A조)신경재생급지체공능정황량호,효과접근우자체신경교접조(C조),명현우우단순거세포신경기막관조(B조).결론 제대간충질간세포복합거세포신경기막관구건적조직공정신경가유효촉진대서좌골신경결손(10 mm)적수복.
Objective To explore the feasibility of using human umbilical cord derived mesenchymal stem cells as seed cells to repair sciatic nerve defects of rats by tissue engineering methods. Methods Mesenchymal stem cells from human umbilical cord were cultured and induced into neuron-liked cells,which were co-cultured with acellular basal lamina tube to construct tissue engineering nerve;models of sciatic nerve defects 10 mm in length were set up with thirty healthy adult SD rats and were divided randomly into 3 groups:tissue engineering nerve group (group A, compound of human umbilical cord derived mesenchymal stem cells and acellular basal lamina tube), pure acellular basal lamina tube group (group B), and autogenous nerve bridging group (group C). Evaluation of electrophysiological and histological results was carried out 10 weeks after operation. Results The engineering nerve group had good result in nerve regeneration which was close to the effect of autogenous nerve transfer group (group A), and much better than the effect of pure acellular basal lamina tube group. Conclusion Engineering nerves from human umbilical cord derived mesenchymal stem cells can effectively repair 10 mm defects of sciatic nerve.
