中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2011年
4期
377-381
,共5页
神经胶质瘤%免疫组织化学%Nanog%CD133%GFAP
神經膠質瘤%免疫組織化學%Nanog%CD133%GFAP
신경효질류%면역조직화학%Nanog%CD133%GFAP
Glioma%Immunohistochemistry%Nanog%CD133%GFAP
目的 研究人脑胶质瘤中Nanog基因与脑肿瘤干细胞标记物CD133、胶质瘤分化标记物神经胶质纤维酸性蛋白(GFAP)之间表达的关系,并探讨Nanog的表达在脑胶质瘤发生发展中的意义.方法 取安徽医科大学附属省立医院神经外科自2009年1月至2009月9月手术切除的人脑胶质瘤组织标本60例,其中组织学分级Ⅱ级19例,Ⅲ级23例,Ⅳ级18例,采用免疫组织化学双重染色检测Nanog/CD133和Nanog/GFAP的表达,比较不同级别胶质瘤Nanog+细胞、CD133+细胞、GFAP+细胞、Nanog+/CD133+细胞和Nanog+/FAP+细胞所占百分比并分析其相关性.结果 不同级别胶质瘤中Nanog+细胞、CD133+细胞、Nanog+/CD133+细胞百分比差异均有统计学意义(P＜0.05),Ⅲ、Ⅳ级胶质瘤中Nanog+细胞、CD133+细胞、Nanog+/CD133+细胞表达高于Ⅱ级,差异有统计学意义(P＜0.05);Nanog+表达和CD133+表达呈正相关关系(r=0.904,P=0.000),Nanog+/CD133+表达亦与CD133+表达呈正相关关系(r=0.823,P=0.000);不同级别胶质瘤GFAP+细胞百分比差异有统计学意义(P＜0.05),Ⅲ、Ⅳ级胶质瘤中GFAP+细胞表达低于Ⅱ级,差异有统计学意义(P＜0.05);不同级别胶质瘤Nanog+/GFAP+细胞百分比差异无统计学意义(P＞0.05).结论 Nanog与干细胞标记物CD133之间具有明显的相关性,在细胞水平上存在显著的共表达.Nanog可能成为胶质瘤干细胞标记物的一个指标,并在维持肿瘤干细胞未分化的状态中起到至关重要的作用.
目的 研究人腦膠質瘤中Nanog基因與腦腫瘤榦細胞標記物CD133、膠質瘤分化標記物神經膠質纖維痠性蛋白(GFAP)之間錶達的關繫,併探討Nanog的錶達在腦膠質瘤髮生髮展中的意義.方法 取安徽醫科大學附屬省立醫院神經外科自2009年1月至2009月9月手術切除的人腦膠質瘤組織標本60例,其中組織學分級Ⅱ級19例,Ⅲ級23例,Ⅳ級18例,採用免疫組織化學雙重染色檢測Nanog/CD133和Nanog/GFAP的錶達,比較不同級彆膠質瘤Nanog+細胞、CD133+細胞、GFAP+細胞、Nanog+/CD133+細胞和Nanog+/FAP+細胞所佔百分比併分析其相關性.結果 不同級彆膠質瘤中Nanog+細胞、CD133+細胞、Nanog+/CD133+細胞百分比差異均有統計學意義(P＜0.05),Ⅲ、Ⅳ級膠質瘤中Nanog+細胞、CD133+細胞、Nanog+/CD133+細胞錶達高于Ⅱ級,差異有統計學意義(P＜0.05);Nanog+錶達和CD133+錶達呈正相關關繫(r=0.904,P=0.000),Nanog+/CD133+錶達亦與CD133+錶達呈正相關關繫(r=0.823,P=0.000);不同級彆膠質瘤GFAP+細胞百分比差異有統計學意義(P＜0.05),Ⅲ、Ⅳ級膠質瘤中GFAP+細胞錶達低于Ⅱ級,差異有統計學意義(P＜0.05);不同級彆膠質瘤Nanog+/GFAP+細胞百分比差異無統計學意義(P＞0.05).結論 Nanog與榦細胞標記物CD133之間具有明顯的相關性,在細胞水平上存在顯著的共錶達.Nanog可能成為膠質瘤榦細胞標記物的一箇指標,併在維持腫瘤榦細胞未分化的狀態中起到至關重要的作用.
목적 연구인뇌효질류중Nanog기인여뇌종류간세포표기물CD133、효질류분화표기물신경효질섬유산성단백(GFAP)지간표체적관계,병탐토Nanog적표체재뇌효질류발생발전중적의의.방법 취안휘의과대학부속성립의원신경외과자2009년1월지2009월9월수술절제적인뇌효질류조직표본60례,기중조직학분급Ⅱ급19례,Ⅲ급23례,Ⅳ급18례,채용면역조직화학쌍중염색검측Nanog/CD133화Nanog/GFAP적표체,비교불동급별효질류Nanog+세포、CD133+세포、GFAP+세포、Nanog+/CD133+세포화Nanog+/FAP+세포소점백분비병분석기상관성.결과 불동급별효질류중Nanog+세포、CD133+세포、Nanog+/CD133+세포백분비차이균유통계학의의(P＜0.05),Ⅲ、Ⅳ급효질류중Nanog+세포、CD133+세포、Nanog+/CD133+세포표체고우Ⅱ급,차이유통계학의의(P＜0.05);Nanog+표체화CD133+표체정정상관관계(r=0.904,P=0.000),Nanog+/CD133+표체역여CD133+표체정정상관관계(r=0.823,P=0.000);불동급별효질류GFAP+세포백분비차이유통계학의의(P＜0.05),Ⅲ、Ⅳ급효질류중GFAP+세포표체저우Ⅱ급,차이유통계학의의(P＜0.05);불동급별효질류Nanog+/GFAP+세포백분비차이무통계학의의(P＞0.05).결론 Nanog여간세포표기물CD133지간구유명현적상관성,재세포수평상존재현저적공표체.Nanog가능성위효질류간세포표기물적일개지표,병재유지종류간세포미분화적상태중기도지관중요적작용.
Objective To study the co-expressions of human glioma transcription factor (Nanog gene) with brain tumor stem cell marker (CD133) and glioma differentiation marker (GFAP), and demonstrate the significance of Nanog gene in the development of glioma. Methods The double-labeled of Nanog/CD133 and Nanog/GFAP were detected by immunohistochemical double staining in 60 cases of human gliomas (19 with grade Ⅱ, 23 with grade Ⅲ and 18 with grade Ⅳ according to WHO 2000 classification of nervous system tumors). The percentages of Nanog+ cells, CD133+ cells,GFAP+ cells, Nanog+/CD133+ cells and Nanog+/GFAP+ cells in glioma tissues of different grades were calculated and compared. Results The percentages of Nanog+, CD133+, and Nanog+/CD133+ cells in different pathological levels of gliomas were significantly different (P＜0.05), and the expressions of Nanog+, CD133+, and Nanog+/CD133+ in gliomas of grade Ⅲ and Ⅳ were significantly higher than those in glioma of grade Ⅱ (P＜0.05). The Nanog+ expression and CD133+ expression were positively correlated (r=0.904, P=0.000), and the Nanog+/CD133+ expression and CD133+ expression were positively related too (r=0.823, P=0.000). The percentages of GFAP+ cells in different pathological levels of gliomas were significantly different (P＜0.05), and the expression of GFAP+ in gliomas of grade Ⅲ and Ⅳ were significantly higher than that in glioma of grade Ⅱ (P＜0.05). No statistical significance of percentage of Nanog+/GFAP+ cells was noted in different pathological levels of gliomas (P＞0.05).Conclusion The Nanog gene and stem cell marker CD 133 has a significant correlation, and they enjoy significant co-expressions in cellular level. Nanog gene may be a marker of glioma stem cells, and plays a vital role in maintaining the cancer stem cells under the undifferentiated state.
