中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
14期
947-951
,共5页
周雯慧%胡卫东%吴洲清%郑新民%汪必成
週雯慧%鬍衛東%吳洲清%鄭新民%汪必成
주문혜%호위동%오주청%정신민%왕필성
前列腺肿瘤%CXCR16/CXCR6%转移
前列腺腫瘤%CXCR16/CXCR6%轉移
전렬선종류%CXCR16/CXCR6%전이
Prostate neoplasms%CXCR16 /CXCR6%Metastasis
目的 探讨趋化因子轴CXCL16/CXCR6在人前列腺癌转移机制中的作用.方法 采用免疫组织化学技术分析CXCL16/CXCR6蛋白在人前列腺癌和骨组织的表达;逆转录聚合酶链反应、免疫细胞化学技术分析CXCR6在人前列腺癌细胞系PC3和LNCap中的表达;迁移、侵袭试验分析外源性CXCL16对Pc3和LNCap细胞体外侵袭能力的调节作用.结果 人前列腺癌组织表达CXCR6蛋白,不表达CXCL16蛋白;正常骨组织表达CXCL16蛋白;PC3和LNCap细胞在基因和蛋白水平表达CXCR6,其mRNA相对转录水平分别为0.38±0.054、0.41±0.019;经过外源性CXCL16处理后,PC3和LNCap的体外迁移、侵袭能力明显提高(侵袭细胞数分别为211.50±5.60、89.25±3.31).且CXCL12或CXCR4中和抗体不能阻断CXCL16的促侵袭作用.结论 CXCL16/CXCR6可能是前列腺癌骨转移的另一种独立趋化因子轴.
目的 探討趨化因子軸CXCL16/CXCR6在人前列腺癌轉移機製中的作用.方法 採用免疫組織化學技術分析CXCL16/CXCR6蛋白在人前列腺癌和骨組織的錶達;逆轉錄聚閤酶鏈反應、免疫細胞化學技術分析CXCR6在人前列腺癌細胞繫PC3和LNCap中的錶達;遷移、侵襲試驗分析外源性CXCL16對Pc3和LNCap細胞體外侵襲能力的調節作用.結果 人前列腺癌組織錶達CXCR6蛋白,不錶達CXCL16蛋白;正常骨組織錶達CXCL16蛋白;PC3和LNCap細胞在基因和蛋白水平錶達CXCR6,其mRNA相對轉錄水平分彆為0.38±0.054、0.41±0.019;經過外源性CXCL16處理後,PC3和LNCap的體外遷移、侵襲能力明顯提高(侵襲細胞數分彆為211.50±5.60、89.25±3.31).且CXCL12或CXCR4中和抗體不能阻斷CXCL16的促侵襲作用.結論 CXCL16/CXCR6可能是前列腺癌骨轉移的另一種獨立趨化因子軸.
목적 탐토추화인자축CXCL16/CXCR6재인전렬선암전이궤제중적작용.방법 채용면역조직화학기술분석CXCL16/CXCR6단백재인전렬선암화골조직적표체;역전록취합매련반응、면역세포화학기술분석CXCR6재인전렬선암세포계PC3화LNCap중적표체;천이、침습시험분석외원성CXCL16대Pc3화LNCap세포체외침습능력적조절작용.결과 인전렬선암조직표체CXCR6단백,불표체CXCL16단백;정상골조직표체CXCL16단백;PC3화LNCap세포재기인화단백수평표체CXCR6,기mRNA상대전록수평분별위0.38±0.054、0.41±0.019;경과외원성CXCL16처리후,PC3화LNCap적체외천이、침습능력명현제고(침습세포수분별위211.50±5.60、89.25±3.31).차CXCL12혹CXCR4중화항체불능조단CXCL16적촉침습작용.결론 CXCL16/CXCR6가능시전렬선암골전이적령일충독립추화인자축.
Objective To explore the roles of chemokine CXCL16 and its receptor CXCR6 in the directional invasion of human prostate cancer ( PCa). Methods The expression of CXCL16/CXCR6 in PCa samples and osseous tissues was determined by immunohistochemistry. The expression of CXCR6 in PC3 and LNCap cells was determined by reverse transcription-polymerase chain reaction ( RT-PCR ) and immunocytochemistry. Then the effects of CXCL16 upon the migration and invasion of human PC3 and LNCap cells were examined by Matrigel invasion assay. Results The expression of CXCR6 protein was detected in all clinical PCa samples. But no CXCL16 protein was detected. Positive CXCL16 expression was observed in human osseous tissues. Both PC3 and LNCap cells expressed CXCR6 mRNA (0. 38 ± 0. 054 vs 0.41 ±0.019 respectively) and protein. In addition, CXCL16 could promote the in vitro migration and invasion of PC3 and LNCap cell lines (invading cells 211.50 ±5. 60 vs 89.25 ±3.31 respectively). Such a promoting effect of CXCL16 could not be blocked influenced by antiCXCL12 or antiCXCR4. Conclusion CXCL16/CXCR6 axis may be another independent chemokine factor playing a significant role in the metastasis of prostate cancer.
