CAJ | 학술논문

首次从夹竹桃中克隆得到苯丙氨酸解氨酶(PAL)基因cDNA片段,并命名为NoPAL1,GenBank登录号为AY747677,这也是首次从夹竹桃花青苷代谢途径中克隆得到的基因.NoPAL1长866个bp,编码289个氨基酸.通过核苷酸和蛋白质序列多重比较发现NoPAL1与其他植物的PAL基因高度同源.NoPAL1编码的蛋白质序列包含与水稻、玉米PAL蛋白质相同的脱氨基位点和催化活性位点.PAL系统进化树表明,NoPAL1与乔木类植物的PAL基因聚类关系最近.克隆NoPAL1基因为利用基因工程技术来调控夹竹桃花青苷的代谢从而调控夹竹桃花的颜色提供了基因资源.
수차종협죽도중극륭득도분병안산해안매(PAL)기인cDNA편단,병명명위NoPAL1,GenBank등록호위AY747677,저야시수차종협죽도화청감대사도경중극륭득도적기인.NoPAL1장866개bp,편마289개안기산.통과핵감산화단백질서렬다중비교발현NoPAL1여기타식물적PAL기인고도동원.NoPAL1편마적단백질서렬포함여수도、옥미PAL단백질상동적탈안기위점화최화활성위점.PAL계통진화수표명,NoPAL1여교목류식물적PAL기인취류관계최근.극륭NoPAL1기인위이용기인공정기술래조공협죽도화청감적대사종이조공협죽도화적안색제공료기인자원.
A phenylalanine ammonia-lyase(PAL) gene was clone from Nerium oleander for the first time and it was also the first cloned gene involved in anthocyanin metabolic pathway in N. oleander. The PAL cDNA fragment of N. oleander (designated as NoPAL1 ) was 866bp and it encoded a 289 amino acid protein. NoPAL1 was found to have extensive homology with those of other plant PAL genes via multiple alignments. The domination sites and catalytic active sites in PAL protein of Oryza sativa and Zea mays were also found in NoPAL1. Phylogenetic tree analysis revealed that NoPAL1 had closer relationship with PALs from arbor plants than from other plants. NoPAL1 is a useful tool to study the regulation of anthocyanis metabolism in N. oleander.