癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2009年
6期
422-425
,共4页
梁婧%黄朴%傅文宇%徐立红
樑婧%黃樸%傅文宇%徐立紅
량청%황박%부문우%서립홍
蛋白磷酸酶2A%微囊藻毒素%mRNA%实时定量PCR
蛋白燐痠酶2A%微囊藻毒素%mRNA%實時定量PCR
단백린산매2A%미낭조독소%mRNA%실시정량PCR
protein phosphotase 2A%microcystin LR%mRNA%real time PCR
背景与目的:研究微囊藻毒素LR(microeystin LR,MCLR)对正常人羊膜细胞FL和肝实质细胞HL7702蛋白磷酸酶2A(PP2A)各亚基mRNA表达的影响.材料与方法:FL和HL7702经100 nmol/L MCLR作用24 h后,采用实时定量PCR方法检测PP2A各亚基mRNA表达的变化.实验并设溶剂对照组. 结果:FL细胞PP2A各亚基mRNA表达部分上升,部分下降;而HL7702细胞PP2A各亚基mRNA表达均上升. 结论:MCLR对肝来源细胞的特异性作用可能与其肝脏毒性有关.
揹景與目的:研究微囊藻毒素LR(microeystin LR,MCLR)對正常人羊膜細胞FL和肝實質細胞HL7702蛋白燐痠酶2A(PP2A)各亞基mRNA錶達的影響.材料與方法:FL和HL7702經100 nmol/L MCLR作用24 h後,採用實時定量PCR方法檢測PP2A各亞基mRNA錶達的變化.實驗併設溶劑對照組. 結果:FL細胞PP2A各亞基mRNA錶達部分上升,部分下降;而HL7702細胞PP2A各亞基mRNA錶達均上升. 結論:MCLR對肝來源細胞的特異性作用可能與其肝髒毒性有關.
배경여목적:연구미낭조독소LR(microeystin LR,MCLR)대정상인양막세포FL화간실질세포HL7702단백린산매2A(PP2A)각아기mRNA표체적영향.재료여방법:FL화HL7702경100 nmol/L MCLR작용24 h후,채용실시정량PCR방법검측PP2A각아기mRNA표체적변화.실험병설용제대조조. 결과:FL세포PP2A각아기mRNA표체부분상승,부분하강;이HL7702세포PP2A각아기mRNA표체균상승. 결론:MCLR대간래원세포적특이성작용가능여기간장독성유관.
BACKGROUND AND AIM: To investigate the effects of microcystin LR on the mRNA expression of protein phosphotase 2A subunits of human amniotic (FL) cells and human hepatocyte HL7702 cells. MATERIALS AND METHODS: After treated with 100 nmol/L MCLR for 24 h, the mRNA expression of protein phosphotase 2A subunits of human amniotic cells and human hepatocyte cells was measured by real time PCR. RESULTS: The changes of subunits expression were diverse in human amniotic cells but increased significantly in human hepatocyte cells. CONCLUSION: The special effects of MCLR on HL7702 cells could be account for its hepatotoxicity.
