CAJ | 학술논문

背景:已经证实大型海藻多糖-琼脂糖改性物具有医学应用价值.目的:以活化降解琼脂糖,明胶可喷涂溶液为敷料,进行皮肤再生实验,探讨琼脂糖改性物作为皮肤再生修复敷料的可能性.方法:将活化琼脂糖和明胶按照一定配比制成总浓度3%的混合水溶液,过滤膜除菌,配制成活化琼脂糖(琼脂糖降解8 h)和明胶将解的,并配制成1:0,1:1,1:2,1:3不同比例.取家兔4只,每只兔子背部选取对称点4个,共取16个实验点,分别将1:1,1:2,1:3比例活化琼脂糖,明胶可喷涂凝胶喷涂于创面个4处;并以喷涂活化琼脂糖2处,单纯明胶2处做对照.术后4周观察喷涂敷料修复创面效果.结果与结论:术后7 d,两种降解时间的活化琼脂糖和明胶配比1:3的覆盖膜已经破裂,另外3个比例仍完好,4种比例创口均没有明显的感染发炎迹象,对比后发现敷料配比为1:2的伤口愈合的最快.明胶的加入对伤口愈合的促进作用很明显.苏木精-伊红染色显示,皮肤创伤经活化降解琼脂糖/明胶复合物修复后的组织与正常组织类似.证实这类琼脂糖改性物具有辅助皮肤再生的功能.
배경:이경증실대형해조다당-경지당개성물구유의학응용개치.목적:이활화강해경지당,명효가분도용액위부료,진행피부재생실험,탐토경지당개성물작위피부재생수복부료적가능성.방법:장활화경지당화명효안조일정배비제성총농도3%적혼합수용액,과려막제균,배제성활화경지당(경지당강해8 h)화명효장해적,병배제성1:0,1:1,1:2,1:3불동비례.취가토4지,매지토자배부선취대칭점4개,공취16개실험점,분별장1:1,1:2,1:3비례활화경지당,명효가분도응효분도우창면개4처;병이분도활화경지당2처,단순명효2처주대조.술후4주관찰분도부료수복창면효과.결과여결론:술후7 d,량충강해시간적활화경지당화명효배비1:3적복개막이경파렬,령외3개비례잉완호,4충비례창구균몰유명현적감염발염적상,대비후발현부료배비위1:2적상구유합적최쾌.명효적가입대상구유합적촉진작용흔명현.소목정-이홍염색현시,피부창상경활화강해경지당/명효복합물수복후적조직여정상조직유사.증실저류경지당개성물구유보조피부재생적공능.
BACKGROUND: It has vedfied that seaweed polysacchande-agarose modifiers have their medical application value. OBJECTIVE: To perform skin regeneration trials using sprayable activated agarose/gelatin solution, and to explore the possibility of egarose modifier as skin dressing for skin regeneration. METHODS: To prepare 3% sprayable mixture with the dissolved activated agarose and gelatin at a certain ratio, and then filtrated with millopore for sterilization to prepare activated agarose (egarose degradation for 8 hours) and gelatin degradation, and made into different ratios (1: 0, 1: 1, 1: 2, 1: 3). A total of four rabbits were obtained, and four sites were selected on the back of each rabbit, totally 16 experimental sites. The sprayable activated agarose/gelatin mixture (1: 1, 1: 2, 1: 3) was directly sprayed on the four lesion sites. Sprayable activated agarose for two sites and simple gelatin for two sites served as controls. The effects of the wounds sprayed with,dressing were observed at 4 weeks following surgery. RESULTS AND CONCLUSION: At 7 days following surgery, the cover film had broken in mixture of activated agarose and gelatin at 1: 3, and remaining three were intact. No infection or inflammation occurred in wound of four ratios. Following comparison, the wound was rapidly healed in 1: 2 ratio dressing. The additional gelatin showed promoting effects on wound healing significantly. Hematoxylin-eosin staining demonstrated that skin with the dressing was similar to autologous skin, which verified that sprayable activated agarose/gelatin have a premise in skin regeneration.