中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2010年
9期
909-915
,共7页
肖雄箭%林建东%蔡毅%张贝蕾%林辉
肖雄箭%林建東%蔡毅%張貝蕾%林輝
초웅전%림건동%채의%장패뢰%림휘
脓毒症%肾%肾衰竭%基因表达%基因芯片
膿毒癥%腎%腎衰竭%基因錶達%基因芯片
농독증%신%신쇠갈%기인표체%기인심편
Sepsis%Kidney%Renal failure%Gene expression%Gene chips
目的 采用基因芯片技术检测分析脓毒症晚期(24 h)肾组织基因表达谱,在基因水平上为脓毒症发病机制提供参考依据.方法 参照盲肠结扎穿孔术(cecal ligation puncture,CLP)制备大鼠脓毒症模型,30只Wistar大鼠随机分(随机数字法)为脓毒症组和对照组.观察两组动物肾功能指标的差异,应用透射电镜观察脓毒症晚期(24 h)大鼠肾组织病理字政变;采用含有22 107个大鼠基因cDNA克隆的表达谱基因芯片检测分析肾组织在脓毒症晚期(24 h)的基因表达,并应用计算机软件筛选出差异表达的基因.采用SPSS 11.0软件进行统计学分析,实验中测得的肾功能指标以均数±标准差((-x)±s)表示,组间比较用两样本t检验,以P＜0.05为差异具有统计学意义.结果 术后24 h脓毒症组尿素氮(BUN)、肌酐(Cr)水平明显高于对照组(P＜0.01),肾组织电镜检查结果提示制模成功.与对照组比较,脓毒症组大鼠肾组织有325个基因出现差异表达,在已知功能基因中,表达上调者100个,表达下调者64个,按照生物学功能分类,主要涉及物质能量代谢、免疫反应、细胞信号转导、细胞凋亡、离子通道和生长因子等方面.结论 脓毒症晚期大鼠肾组织出现一系列基因表达的异常,基因芯片检测技术是研究脓毒症基因机制的一个手段.
目的 採用基因芯片技術檢測分析膿毒癥晚期(24 h)腎組織基因錶達譜,在基因水平上為膿毒癥髮病機製提供參攷依據.方法 參照盲腸結扎穿孔術(cecal ligation puncture,CLP)製備大鼠膿毒癥模型,30隻Wistar大鼠隨機分(隨機數字法)為膿毒癥組和對照組.觀察兩組動物腎功能指標的差異,應用透射電鏡觀察膿毒癥晚期(24 h)大鼠腎組織病理字政變;採用含有22 107箇大鼠基因cDNA剋隆的錶達譜基因芯片檢測分析腎組織在膿毒癥晚期(24 h)的基因錶達,併應用計算機軟件篩選齣差異錶達的基因.採用SPSS 11.0軟件進行統計學分析,實驗中測得的腎功能指標以均數±標準差((-x)±s)錶示,組間比較用兩樣本t檢驗,以P＜0.05為差異具有統計學意義.結果 術後24 h膿毒癥組尿素氮(BUN)、肌酐(Cr)水平明顯高于對照組(P＜0.01),腎組織電鏡檢查結果提示製模成功.與對照組比較,膿毒癥組大鼠腎組織有325箇基因齣現差異錶達,在已知功能基因中,錶達上調者100箇,錶達下調者64箇,按照生物學功能分類,主要涉及物質能量代謝、免疫反應、細胞信號轉導、細胞凋亡、離子通道和生長因子等方麵.結論 膿毒癥晚期大鼠腎組織齣現一繫列基因錶達的異常,基因芯片檢測技術是研究膿毒癥基因機製的一箇手段.
목적 채용기인심편기술검측분석농독증만기(24 h)신조직기인표체보,재기인수평상위농독증발병궤제제공삼고의거.방법 삼조맹장결찰천공술(cecal ligation puncture,CLP)제비대서농독증모형,30지Wistar대서수궤분(수궤수자법)위농독증조화대조조.관찰량조동물신공능지표적차이,응용투사전경관찰농독증만기(24 h)대서신조직병리자정변;채용함유22 107개대서기인cDNA극륭적표체보기인심편검측분석신조직재농독증만기(24 h)적기인표체,병응용계산궤연건사선출차이표체적기인.채용SPSS 11.0연건진행통계학분석,실험중측득적신공능지표이균수±표준차((-x)±s)표시,조간비교용량양본t검험,이P＜0.05위차이구유통계학의의.결과 술후24 h농독증조뇨소담(BUN)、기항(Cr)수평명현고우대조조(P＜0.01),신조직전경검사결과제시제모성공.여대조조비교,농독증조대서신조직유325개기인출현차이표체,재이지공능기인중,표체상조자100개,표체하조자64개,안조생물학공능분류,주요섭급물질능량대사、면역반응、세포신호전도、세포조망、리자통도화생장인자등방면.결론 농독증만기대서신조직출현일계렬기인표체적이상,기인심편검측기술시연구농독증기인궤제적일개수단.
Objective To investigate the gene-expression profile in kidney of rats during late sepsis (24hours) by using microarray technology in order to offer some clue to revealing the pathogenetic mechanism of sepsis at gene level. Method A total of 30 Wistar rats were selected and divided into model group and control group randomly(random number). The rats of control group were sham operated and the rats of model group received cecal ligature and puncture (CLP) operation. The biomarkers of renal function were assayed and the histopathological changes of kidney in rats were observed under transmission electron microscope 24 hours after operation. Gene chips containing 22 107 rat-genes cDNA were used to exmine gene-expression in kidney of septic rats to sieve the genes with different expressions with software. Data were analyzed by using SPSS version 11.0 software package.Statistical analyses of two independent samples carried out by using t -test. Results Compared with the control group, the levels of blood urea nitrogen (BUN) and creatinine (Cr) of sepsis group were higher (P ＜ 0.01 ). The histopathological changes in kidney of rats demonstrated the establishment of sepsis model successful 24 hours later.Compared with the control group, there were 325 genes with differential expression in model group. Among the known-functional genes, there were 100 up-regulated and 64 down-regulated. Sorted by biological function, the genes were mainly related to metabolism, immunoresponse, cellular signal transduction, apoptosis, ion channel,growth factor and so on. Conclusions A sequence of genes expressed differentially in kidney of rats with late sepsis. Microarray technology played an important role in the research into sepsis mechanisms.
