国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2011年
8期
597-600
,共4页
凝血酶%永生化人支气管上皮细胞%活性氧簇%血小板源生长因子-AB
凝血酶%永生化人支氣管上皮細胞%活性氧簇%血小闆源生長因子-AB
응혈매%영생화인지기관상피세포%활성양족%혈소판원생장인자-AB
Thrombin%Immortalized human bronchial epithelial cells%Reactive oxygen species%Platelet-derived growth factor-AB
目的 探讨凝血酶对永生化人支气管上皮细胞(BEP2D细胞)内活性氧产生及血小板源生长因子-AB(PDGF-AB)分泌的影响.方法 不同浓度凝血酶刺激指数生长期的BEP2D细胞,通过检测氧化型氢化乙啶及二氯荧光素荧光强度测定活性氧簇含量变化.采用双抗体夹心ELISA法检测BEP2D细胞分泌PDGF-AB的变化.结果 随着凝血酶浓度增加,反应体系中活性氧明显升高,且有明显的剂量反应关系.凝血酶处理组BEP2D细胞上清液中PDGF-AB含量较对照组上清液显著升高[(770.33+24.29)ng/L vs(117.42±10.85)ng/L,P＜0.01].凝血酶在一定范围内有剂量反应关系.10 U/ml凝血酶刺激48 h后BEP2D细胞分泌PDGF-AB量最大[(817.63+22.53)ng/L].结论 凝血酶既可以引起细胞内氧化应激导致基因毒性,又可以通过刺激BEP2D细胞分泌PDGF-AB发挥自分泌及旁分泌作用.
目的 探討凝血酶對永生化人支氣管上皮細胞(BEP2D細胞)內活性氧產生及血小闆源生長因子-AB(PDGF-AB)分泌的影響.方法 不同濃度凝血酶刺激指數生長期的BEP2D細胞,通過檢測氧化型氫化乙啶及二氯熒光素熒光彊度測定活性氧簇含量變化.採用雙抗體夾心ELISA法檢測BEP2D細胞分泌PDGF-AB的變化.結果 隨著凝血酶濃度增加,反應體繫中活性氧明顯升高,且有明顯的劑量反應關繫.凝血酶處理組BEP2D細胞上清液中PDGF-AB含量較對照組上清液顯著升高[(770.33+24.29)ng/L vs(117.42±10.85)ng/L,P＜0.01].凝血酶在一定範圍內有劑量反應關繫.10 U/ml凝血酶刺激48 h後BEP2D細胞分泌PDGF-AB量最大[(817.63+22.53)ng/L].結論 凝血酶既可以引起細胞內氧化應激導緻基因毒性,又可以通過刺激BEP2D細胞分泌PDGF-AB髮揮自分泌及徬分泌作用.
목적 탐토응혈매대영생화인지기관상피세포(BEP2D세포)내활성양산생급혈소판원생장인자-AB(PDGF-AB)분비적영향.방법 불동농도응혈매자격지수생장기적BEP2D세포,통과검측양화형경화을정급이록형광소형광강도측정활성양족함량변화.채용쌍항체협심ELISA법검측BEP2D세포분비PDGF-AB적변화.결과 수착응혈매농도증가,반응체계중활성양명현승고,차유명현적제량반응관계.응혈매처리조BEP2D세포상청액중PDGF-AB함량교대조조상청액현저승고[(770.33+24.29)ng/L vs(117.42±10.85)ng/L,P＜0.01].응혈매재일정범위내유제량반응관계.10 U/ml응혈매자격48 h후BEP2D세포분비PDGF-AB량최대[(817.63+22.53)ng/L].결론 응혈매기가이인기세포내양화응격도치기인독성,우가이통과자격BEP2D세포분비PDGF-AB발휘자분비급방분비작용.
Objective To investigate the effects of thrombin on reactive oxygen species and plateletderived growth factor-AB (PDGF-AB) in immortalized human bronchial epithelial cells (BEP2D cells).Methods BEP2D cells at exponential growth phase were stimulated by different concentrations of thrombin. The content of active oxygen species was determined by detecting the fluorescence intensity of hydroxyethidium and dichlorofluorescein. The changes of PDGF-AB were measured by double antibody sandwich ELISA assay. Results With the increase of the thrombin concentration, reactive oxygen species significantly increased, and there was a dose-response relationship. The concentration of PDGF-AB in culture supematants in thrombin-treated group was higher than that in control group [(770. 33 +24. 29 )ng/L vs (117. 42+ 10.85) ng/L, P ＜ 0. 01], and there was a dose-response relationship. The concentration of PDGF-AB was maximal [(817.63 +22.53) ng/L] when BEP2D cells were stimulated with 10 U/ml thrombin for 48 hours. Conclusions Thrombin can induce cellular oxidative stress to lead to genotoxicity,and play autocrine and paracrine role by stimulating the secretion of PDGF-AB in BEP2D cells.
