CAJ | 학술논문

目的探讨促红细胞生成素对大鼠急性肾小管坏死的防治作用.方法按10 mL·kg-1肌肉注射甘油建立大鼠急性肾小管坏死模型.30只Wistar大鼠随机(随机数字法)分为正常组、模型组、治疗组.治疗组予促红细胞生成素(1000 IU·kg-1)尾静脉注射,实验结束时检测各组大鼠血尿素氮(Bun)、血肌酐(Scr)、尿渗量、尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)、尿β2微球蛋白(β2-MG),肾组织匀浆丙二醇(MDA)和超氧化物歧化酶(SOD),并进行肾脏组织学观察.结果与模型组比较,治疗组大鼠Bun、Scr、尿NAG、尿β2-MG、肾组织匀浆MDA明显下降,而尿渗量、肾组织匀浆SOD升高,肾脏病理改变好转.结论促红细胞生成素可能通过抗氧自由基损伤,提高内源性抗氧化能力达到对急性肾小管坏死大鼠肾脏的部分保护作用.
목적 탐토촉홍세포생성소대대서급성신소관배사적방치작용.방법 안10 mL·kg-1기육주사감유건립대서급성신소관배사모형.30지Wistar대서수궤(수궤수자법)분위정상조、모형조、치료조.치료조여촉홍세포생성소(1000 IU·kg-1)미정맥주사,실험결속시검측각조대서혈뇨소담(Bun)、혈기항(Scr)、뇨삼량、뇨N-을선-β-D-안기포도당감매(NAG)、뇨β2미구단백(β2-MG),신조직균장병이순(MDA)화초양화물기화매(SOD),병진행신장조직학관찰.결과 여모형조비교,치료조대서Bun、Scr、뇨NAG、뇨β2-MG、신조직균장MDA명현하강,이뇨삼량、신조직균장SOD승고,신장병리개변호전.결론 촉홍세포생성소가능통과항양자유기손상,제고내원성항양화능력체도대급성신소관배사대서신장적부분보호작용.
Objective To study the preventive effects of erythropoietin (EPO) on acute tubular necrosis of kidney in rats. Method The rat models of acute renal tubular necrosis were established with injecting glycerol in dose of 10 mL/kg. Thirty Wistar rats were randomly (random number) divided into control group, model group and EPO treatment group. EPO was administered intravenously into rats of treatment group in a dose of 1000IU/kg. Levels of blood urea nitrogen (BUN) and serum creatinine (Scr), urine osmolality, urine N-acetyl-D-glucosaminidase (NAG), urine β2-microglobulin (β2-MG), tissue MDA and SOD of rats in the three groups were assayed after the experiment. Renal histological examination was also performed. Results Compared with model group, the levels of BUN and Scr, urine osmolality, NAG,β2-MG and tissue MDA in EPO treament group were significantly lower, but urine osmolality and tissue SOD of rats remarkably increased in comparison with model group. EPO also lessened the histological changes in treatment group. Conclusions EPO has some protective effects on acute renal tubular necrosis in rats, which is probably through preventing oxygen free radical damage and elevating endogenous antioxidation potential.