中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
2期
83-86
,共4页
巫丰宏%周旋%张娆%潘梅竹%彭开良
巫豐宏%週鏇%張嬈%潘梅竹%彭開良
무봉굉%주선%장요%반매죽%팽개량
过氯酸铵%甲状腺%甲状腺激素类%甲状腺球蛋白%碘化物过氧化物酶
過氯痠銨%甲狀腺%甲狀腺激素類%甲狀腺毬蛋白%碘化物過氧化物酶
과록산안%갑상선%갑상선격소류%갑상선구단백%전화물과양화물매
Ammonium perchlorate%Thyroid gland%Thyroid hormones%Thyroglobulin%Iodide peroxidase
目的 研究过氯酸铵(AP)对大鼠甲状腺功能及甲状腺球蛋白(Tg)、甲状腺过氧化物酶(TPO)基因mRNA表达的影响.方法 将雄性SD大鼠30只随机分为:正常对照组、低碘组(含碘量:50μg/kg)、AP染毒低(130 mg/kg)、中(260 mg/kg)、高剂量组(520 mg/kg)及AP+高碘组[AP:520 mg/kg,高碘饮水(10 mg/L)],每组5只.经口染毒90 d后处死,放射免疫法测定血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)及促甲状腺激素(TSH)的水平;荧光定量PCR法测定甲状腺球蛋白(Tg)、甲状腺过氧化物酶(TPO)基因mRNA表达水平.结果 AP中剂量和AP高剂量组FT4水平[(9.540±1.327)fmol/ml,(6.509±1.949)fmol/ml)明显低于正常对照组[(13.505±1.276)fmol/ml],差异有统计学意义(P＜0.05,P＜0.01).AP高剂量组的TSH水平[(1.227±0.295)mIU/L]明显高于正常对照组[(0.545±0.282)mIU/L],差异有统计学意义(P＜0.05);各AP剂量组Tg的mRNA表达相对值均明显低于正常对照组,差异有统计学意义(P＜0.01),AP高剂量组TPO基因mRNA表达相对值明显低于正常对照组,差异有统计学意义(P＜0.05).结论 AP可降低大鼠FT3、FT4水平,引起TSH反馈性增高,并明显抑制Tg和TPO基因mRNA的表达;而高碘可以在一定程度上拮抗AP对大鼠甲状腺的毒作用.
目的 研究過氯痠銨(AP)對大鼠甲狀腺功能及甲狀腺毬蛋白(Tg)、甲狀腺過氧化物酶(TPO)基因mRNA錶達的影響.方法 將雄性SD大鼠30隻隨機分為:正常對照組、低碘組(含碘量:50μg/kg)、AP染毒低(130 mg/kg)、中(260 mg/kg)、高劑量組(520 mg/kg)及AP+高碘組[AP:520 mg/kg,高碘飲水(10 mg/L)],每組5隻.經口染毒90 d後處死,放射免疫法測定血清遊離三碘甲狀腺原氨痠(FT3)、遊離甲狀腺素(FT4)及促甲狀腺激素(TSH)的水平;熒光定量PCR法測定甲狀腺毬蛋白(Tg)、甲狀腺過氧化物酶(TPO)基因mRNA錶達水平.結果 AP中劑量和AP高劑量組FT4水平[(9.540±1.327)fmol/ml,(6.509±1.949)fmol/ml)明顯低于正常對照組[(13.505±1.276)fmol/ml],差異有統計學意義(P＜0.05,P＜0.01).AP高劑量組的TSH水平[(1.227±0.295)mIU/L]明顯高于正常對照組[(0.545±0.282)mIU/L],差異有統計學意義(P＜0.05);各AP劑量組Tg的mRNA錶達相對值均明顯低于正常對照組,差異有統計學意義(P＜0.01),AP高劑量組TPO基因mRNA錶達相對值明顯低于正常對照組,差異有統計學意義(P＜0.05).結論 AP可降低大鼠FT3、FT4水平,引起TSH反饋性增高,併明顯抑製Tg和TPO基因mRNA的錶達;而高碘可以在一定程度上拮抗AP對大鼠甲狀腺的毒作用.
목적 연구과록산안(AP)대대서갑상선공능급갑상선구단백(Tg)、갑상선과양화물매(TPO)기인mRNA표체적영향.방법 장웅성SD대서30지수궤분위:정상대조조、저전조(함전량:50μg/kg)、AP염독저(130 mg/kg)、중(260 mg/kg)、고제량조(520 mg/kg)급AP+고전조[AP:520 mg/kg,고전음수(10 mg/L)],매조5지.경구염독90 d후처사,방사면역법측정혈청유리삼전갑상선원안산(FT3)、유리갑상선소(FT4)급촉갑상선격소(TSH)적수평;형광정량PCR법측정갑상선구단백(Tg)、갑상선과양화물매(TPO)기인mRNA표체수평.결과 AP중제량화AP고제량조FT4수평[(9.540±1.327)fmol/ml,(6.509±1.949)fmol/ml)명현저우정상대조조[(13.505±1.276)fmol/ml],차이유통계학의의(P＜0.05,P＜0.01).AP고제량조적TSH수평[(1.227±0.295)mIU/L]명현고우정상대조조[(0.545±0.282)mIU/L],차이유통계학의의(P＜0.05);각AP제량조Tg적mRNA표체상대치균명현저우정상대조조,차이유통계학의의(P＜0.01),AP고제량조TPO기인mRNA표체상대치명현저우정상대조조,차이유통계학의의(P＜0.05).결론 AP가강저대서FT3、FT4수평,인기TSH반궤성증고,병명현억제Tg화TPO기인mRNA적표체;이고전가이재일정정도상길항AP대대서갑상선적독작용.
Objective To investigate the effects of ammonium perchlorate (AP) on thyroid functions and mRNA expression levels of thyroglobulin(Tg) and thyroperoxidase (TPO) genes of rats. Methods Thirty SD male rats were randomly divided into six groups: control group, iodine-deficient group, low dose AP group ( 130 mg/kg), moderate dose AP group (260 mg/kg), high dose AP group (520 mg/kg) and high iodinecombined group. After the rats were exposed orally for 90 days, serum free-thyroxine (FT4), freetriiodothyronine (FT3) and thyroid stimulating hormone (TSH) were measured using radioimmunoassays.mRNA expression levels of thyroglobulin (Tg) and thyroperoxidase (TPO) genes were detected by real-time quantitative PCR. Results Serum FT4 levels in moderate dose AP group and high dose AP group were [(9.540±1.327) fmol/ml] and [(6.509±1.949) fmol/ml] respectively, which were significantly lower than that [ (13.505 ±1.276 ) fmol/ml] in control group (P＜0.05 or P＜0.01). Serum TSH level in high dose AP group was [(1.227±0.295) mIU/L], which was significantly higher than that [(0.545±0.282) mIU/L] in control group (P＜0.05). The mRNA expression levels of thyroglobulin (Tg)gene in all groups exposed to AP were significantly lower than that in control group (P＜0.01). The mRNA expression level of thyroperoxidase (TPO)gene in high dose AP group was significantly higher than that in control group (P＜0.05). Conclusion AP can reduce the serum FT3 and FT4levels of rats, increase the serum TSH level of rats and decrease obviously the mRNA expression levels of Tg and TPO genes. In addition, high iodine can reduce the toxic effects of AP on thyroid gland of rats to some extent.
